Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria.
Redox Rep. 2011;16(2):56-61. doi: 10.1179/174329211X12989133691530.
The antioxidant effect of saponarin, which is the main flavone isolated from Gypsophila trichotoma Wend., and its protection against cocaine hepatotoxicity were investigated in male Wistar rats. The animals were treated with cocaine (40 mg/kg i.p.) alone and also after 3 consecutive days of pretreatment with saponarin (80 mg/kg p.o.). After 18 hours the rats were sacrificed by decapitation. The production of thiobarbituric acid reactive substances, reduced glutathione (GSH) and the activity of the following antioxidant enzymes: catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase were assessed in liver homogenate. Administered alone, cocaine induced significant hepatotoxicity manifested with GSH depletion and reduced antioxidant defences. Saponarin pretreatment, however, decreased cocaine toxicity both by increasing GSH levels and antioxidant enzyme activities. The results of this study proved the antioxidant activity of saponarin and its protective effect against cocaine-induced oxidative stress and hepatotoxicity.
从麦瓶草中分离得到的主要黄酮类化合物——毛蕊异黄酮苷,具有抗氧化作用,本研究旨在探讨其对可卡因肝毒性的保护作用。雄性 Wistar 大鼠腹腔注射可卡因(40mg/kg)单独给药,以及连续 3 天预先给予毛蕊异黄酮苷(80mg/kg,灌胃)后给药。18 小时后,通过断头处死大鼠。在肝匀浆中评估硫代巴比妥酸反应物质、还原型谷胱甘肽(GSH)以及以下抗氧化酶的活性:过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶。可卡因单独给药可导致明显的肝毒性,表现为 GSH 耗竭和抗氧化防御能力降低。然而,毛蕊异黄酮苷预处理通过增加 GSH 水平和抗氧化酶活性,降低了可卡因的毒性。本研究结果证明了毛蕊异黄酮苷的抗氧化活性及其对可卡因诱导的氧化应激和肝毒性的保护作用。