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库拉索芦荟(Aloe barbadensis)干花中的酚类成分及其体外抗氧化能力。

Phenolic constituents in dried flowers of aloe vera (Aloe barbadensis) and their in vitro antioxidative capacity.

作者信息

Keyhanian Shirin, Stahl-Biskup Elisabeth

机构信息

Institute of Pharmacy, Division of Pharmaceutical Biology and Microbiology, University of Hamburg, Hamburg, Germany.

出版信息

Planta Med. 2007 Jun;73(6):599-602. doi: 10.1055/s-2007-967202. Epub 2007 May 22.

DOI:10.1055/s-2007-967202
PMID:17520524
Abstract

The dried flowers from Aloe vera (L.) Burm. f. (Aloe barbadensis Mill.) (Asphodelaceae) were analysed by means of HPLC-DAD and HPLC-MS/MS, verifying chlorogenic, caffeic, 5-P-coumaroylquinic, caffeoylshikimic, 5-feruloylquinic, 5-P-CIS-coumaroylquinic, P-coumaric and ferulic acid as well as luteolin, apigenin, quercetin, kaempferol, isoorientin, isovitexin and their 7-O-glucosides, saponarin and lutonarin. On searching for anthranoids in the flower extract, aloe-emodin as well as the glycosylchromone aloeresin B could be identified. Aloin A and B, the laxative principle of the drug Curaçao-Aloes, are not accumulated in the dried flowers. The polyphenol content of three different batches was 0.73 - 1.01% (+/- 0.05%) and the flavonoid content 0.24 - 0.34% (+/- 0.01%). The hydrophilic antioxidative capacity amounted to 85.7 - 94.9 (+/- 0.5) micromol TEAC/g dried Aloe vera flower and was directly correlated with the polyphenol and flavonoid contents.

摘要

采用高效液相色谱 - 二极管阵列检测法(HPLC - DAD)和高效液相色谱 - 串联质谱法(HPLC - MS/MS)对库拉索芦荟(学名:Aloe vera (L.) Burm. f.,又称巴巴多斯芦荟,百合科)的干花进行分析,验证了其中含有绿原酸、咖啡酸、5 - 对香豆酰奎尼酸、咖啡酰莽草酸、5 - 阿魏酰奎尼酸、5 - 对 - 顺 - 香豆酰奎尼酸、对香豆酸和阿魏酸,以及木犀草素、芹菜素、槲皮素、山奈酚、异荭草素、异牡荆素及其7 - O - 葡萄糖苷、皂草苷和卢托那林。在花提取物中寻找蒽类化合物时,鉴定出芦荟大黄素以及糖基色酮芦荟树脂B。作为药物库拉索芦荟泻药成分的芦荟苷A和B,在干花中并未积累。三个不同批次的多酚含量为0.73 - 1.01%(±0.05%),黄酮含量为0.24 - 0.34%(±0.01%)。亲水性抗氧化能力为85.7 - 94.9(±0.5)微摩尔TEAC/克库拉索芦荟干花,且与多酚和黄酮含量直接相关。

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