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从糙叶败酱中分离得到的瑞香苷对乙酰氨基酚诱导的大鼠肝损伤的肝保护和抗氧化作用。

Hepatoprotective and antioxidant effects of saponarin, isolated from Gypsophila trichotoma Wend. on paracetamol-induced liver damage in rats.

机构信息

Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, 2 Dunav Street, 1000 Sofia, Bulgaria.

出版信息

Biomed Res Int. 2013;2013:757126. doi: 10.1155/2013/757126. Epub 2013 Jun 26.

Abstract

The hepatoprotective potential of saponarin, isolated from Gypsophila trichotoma, was evaluated in vitro/in vivo using a hepatotoxicity model of paracetamol-induced liver injury. In freshly isolated rat hepatocytes, paracetamol (100 μ mol) led to a significant decrease in cell viability, increased LDH leakage, decreased levels of cellular GSH, and elevated MDA quantity. Saponarin (60-0.006 μ g/mL) preincubation, however, significantly ameliorated paracetamol-induced hepatotoxicity in a concentration-dependent manner. The beneficial effect of saponarin was also observed in vivo. Rats were challenged with paracetamol alone (600 mg/kg, i.p.) and after 7-day pretreatment with saponarin (80 mg/kg, oral gavage). Paracetamol toxicity was evidenced by increase in MDA quantity and decrease in cell GSH levels and antioxidant defence system. No changes in phase I enzyme activities of AH and EMND and cytochrome P 450 quantity were detected. Saponarin pretreatment resulted in significant increase in cell antioxidant defence system and GSH levels and decrease in lipid peroxidation. The biochemical changes are in good correlation with the histopathological data. Protective activity of saponarin was similar to the activity of positive control silymarin. On the basis of these results, it can be concluded that saponarin exerts antioxidant and hepatoprotective activity against paracetamol liver injury in vitro/in vivo.

摘要

从婆婆纳中分离得到的瑞香苷的保肝作用,通过对乙酰氨基酚诱导的肝损伤毒性模型进行体外/体内实验进行了评估。在新鲜分离的大鼠肝细胞中,对乙酰氨基酚(100 μmol)导致细胞活力显著下降,LDH 漏出增加,细胞内 GSH 水平降低,MDA 含量升高。然而,瑞香苷(60-0.006 μg/mL)预孵育以浓度依赖性方式显著改善了对乙酰氨基酚诱导的肝毒性。瑞香苷的有益作用也在体内观察到。大鼠单独用对乙酰氨基酚(600 mg/kg,腹腔注射)和用瑞香苷(80 mg/kg,口服灌胃)预处理 7 天后进行挑战。对乙酰氨基酚毒性表现为 MDA 含量增加、细胞 GSH 水平降低和抗氧化防御系统减少。AH 和 EMND 的 I 相酶活性和细胞色素 P 450 数量没有变化。瑞香苷预处理导致细胞抗氧化防御系统和 GSH 水平显著增加,脂质过氧化减少。生化变化与组织病理学数据密切相关。瑞香苷的保护活性与阳性对照水飞蓟素的活性相似。基于这些结果,可以得出结论,瑞香苷在体外/体内对乙酰氨基酚肝损伤具有抗氧化和保肝作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc36/3708395/2ff89c838f0d/BMRI2013-757126.001.jpg

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