Oliveira Lorena Mayana Beserra de, Bevilaqua Claudia Maria Leal, Macedo Iara Tersia Freitas, Morais Selene Maia de, Monteiro Maria Vivina Barros, Campello Claudio Cabral, Ribeiro Wesley Lyeverton Correia, Batista Emanuelle Karine Frota
Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Av. Dedé Brazil 1700, Campus do Itaperi, Fortaleza, CE, Brazil.
Rev Bras Parasitol Vet. 2011 Apr-Jun;20(2):155-60. doi: 10.1590/s1984-29612011000200011.
Tanniferous plants represent a promising alternative for controlling gastrointestinal nematodes of small ruminants. This experiment evaluated the effects of extracts from the leaf and stem of Anadenanthera colubrina, Leucaena leucocephala and Mimosa tenuiflora on larval exsheathment of Haemonchus contortus in vitro and verified the role of tannins in this process. Third-stage larvae of H. contortus were incubated with extracts for 3 hours and were exposed to sodium hypochlorite solution. The extracts were tested at 300 μg.mL(-1) and accompanied by controls: phosphate buffer solution (PBS) and polyvinyl polypyrrolidone (PVPP). The larval exsheathment was evaluated for 60 minutes, and the results were subjected to the Kruskal-Wallis test (p < 0.05). The six extracts blocked larval exsheathment. After PVPP addition, a tannin inhibitor, the exsheathment percentage was similar to the PBS (p > 0.05), except for L. leucocephala and M. tenuiflora leaf extracts. However, pre-incubation with PVPP of these two extracts significantly changed larval exsheathment when compared to the non-treated extracts (p < 0.05). These results suggest that A. colubrina, L. leucocephala and M. tenuiflora could be useful in gastrointestinal nematode control and that tannins are probably the main compounds involved in the observed effects. However, in vivo and toxicological studies should be conducted.
含单宁植物是控制小型反刍动物胃肠道线虫的一种有前景的替代方法。本实验评估了南美猴耳环、银合欢和含羞草叶和茎提取物对体外捻转血矛线虫幼虫脱鞘的影响，并验证了单宁在这一过程中的作用。将捻转血矛线虫的第三期幼虫与提取物孵育3小时，然后暴露于次氯酸钠溶液中。提取物在300μg.mL(-1)浓度下进行测试，并设置对照组：磷酸盐缓冲溶液（PBS）和聚乙烯聚吡咯烷酮（PVPP）。对幼虫脱鞘情况评估60分钟，结果进行Kruskal-Wallis检验（p<0.05）。六种提取物均能阻止幼虫脱鞘。添加单宁抑制剂PVPP后，除银合欢和含羞草叶提取物外，脱鞘百分比与PBS相似（p>0.05）。然而，与未处理的提取物相比，这两种提取物与PVPP预孵育后显著改变了幼虫脱鞘情况（p<0.05）。这些结果表明，南美猴耳环、银合欢和含羞草可能有助于控制胃肠道线虫，单宁可能是观察到的效果的主要相关化合物。然而，应进行体内和毒理学研究。
