Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Brazil.
Vet Parasitol. 2013 Sep 23;196(3-4):341-6. doi: 10.1016/j.vetpar.2013.04.014. Epub 2013 Apr 16.
Anthelmintic resistance has limited the ability to control the gastrointestinal nematodes of small ruminants and has therefore awakened an interest in the study of tanniferous plants as a source of anthelmintics. This study was carried out to evaluate the effect of Mimosa tenuiflora intake, a tanniferous plant that is fed to small ruminants in northeastern Brazil, on the larval establishment of Haemonchus contortus in sheep. In this experiment, 18 nematode-free sheep were divided into three groups (n=6) according to live weight. Group 1 was fed M. tenuiflora leaves; Group 2 was fed M. tenuiflora stems; Group 3 served as the control group and was fed Cynodon dactylon, a plant with low levels of tannins. The animals consumed the plants for 13 days (Day -7 to Day 5). On Day 0, the sheep were experimentally infected with 4500 third-stage H. contortus each. Five days after infection (Day 5), the sheep were slaughtered to count the worm burden and perform a histological analysis of the abomasum. The daily plant intake and the live weight gain of the animals were recorded. The groups that ingested M. tenuiflora leaves and stems consumed less dry matter than did those that ingested C. dactylon (P<0.05). The consumption of M. tenuiflora leaves did not reduce the L3 establishment of H. contortus compared to the control (P>0.05). The intake of M. tenuiflora stems tended toward decreasing larval establishment, but the reduction was not significant (P>0.05). No significant differences were observed in the mucosal cellular response and live weight gain among the groups. These data demonstrated that, with the protocol used, M. tenuiflora has no effect on larval establishment of H. contortus in sheep.
抗蠕虫药物耐药性限制了对小反刍动物胃肠道线虫的控制能力，因此人们对研究单宁植物作为驱虫剂来源产生了兴趣。本研究旨在评估在巴西东北部作为小反刍动物饲料的单宁植物含羞草摄入对绵羊捻转血矛线虫幼虫定植的影响。在这项实验中，根据活体重将 18 只无寄生虫绵羊分为三组（n=6）。第 1 组饲喂含羞草叶片；第 2 组饲喂含羞草茎；第 3 组作为对照组，饲喂低单宁水平的狗牙根。动物连续采食植物 13 天（-7 天至 5 天）。在第 0 天，绵羊实验性感染 4500 条捻转血矛线虫第 3 期幼虫。感染后第 5 天（第 5 天），宰杀绵羊计数虫体负荷并对皱胃进行组织学分析。记录每日植物采食量和动物活体重增加。采食含羞草叶片和茎的组比采食狗牙根的组消耗的干物质更少（P<0.05）。与对照组相比，采食含羞草叶片并未降低 H. contortus 的 L3 定植（P>0.05）。采食含羞草茎有降低幼虫定植的趋势，但差异不显著（P>0.05）。各组黏膜细胞反应和活体重增加无显著差异。这些数据表明，在使用的方案中，含羞草对绵羊捻转血矛线虫幼虫定植没有影响。
