Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3JT, UK.
J Proteomics. 2011 Aug 24;74(9):1573-94. doi: 10.1016/j.jprot.2011.06.002. Epub 2011 Jun 29.
The intestinal helminth parasite, Heligmosomoides polygyrus bakeri offers a tractable experimental model for human hookworm infections such as Ancylostoma duodenale and veterinary parasites such as Haemonchus contortus. Parasite excretory-secretory (ES) products represent the major focus for immunological and biochemical analyses, and contain immunomodulatory molecules responsible for nematode immune evasion. In a proteomic analysis of adult H. polygyrus secretions (termed HES) matched to an extensive transcriptomic dataset, we identified 374 HES proteins by LC-MS/MS, which were distinct from those in somatic extract HEx, comprising 446 identified proteins, confirming selective export of ES proteins. The predominant secreted protein families were proteases (astacins and other metalloproteases, aspartic, cysteine and serine-type proteases), lysozymes, apyrases and acetylcholinesterases. The most abundant products were members of the highly divergent venom allergen-like (VAL) family, related to Ancylostoma secreted protein (ASP); 25 homologues were identified, with VAL-1 and -2 also shown to be associated with the parasite surface. The dominance of VAL proteins is similar to profiles reported for Ancylostoma and Haemonchus ES products. Overall, this study shows that the secretions of H. polygyrus closely parallel those of clinically important GI nematodes, confirming the value of this parasite as a model of helminth infection.
肠道蠕虫寄生虫 Heligmosomoides polygyrus bakeri 为人类钩虫感染（如 Ancylostoma duodenale）和兽医寄生虫（如 Haemonchus contortus）提供了一种可行的实验模型。寄生虫排泄-分泌（ES）产物是免疫和生化分析的主要焦点，其中包含负责线虫免疫逃避的免疫调节分子。在与广泛的转录组数据集相匹配的成年 H. polygyrus 分泌物（称为 HES）的蛋白质组分析中，我们通过 LC-MS/MS 鉴定了 374 种 HES 蛋白，这些蛋白与体细胞提取物 HEx 中的蛋白不同，包含 446 种已鉴定的蛋白，证实 ES 蛋白的选择性输出。主要分泌的蛋白家族是蛋白酶（天冬氨酸蛋白酶和其他金属蛋白酶、天冬氨酸、半胱氨酸和丝氨酸型蛋白酶）、溶菌酶、apyrases 和乙酰胆碱酯酶。最丰富的产物是高度分化的毒液过敏原样（VAL）家族的成员，与 Ancylostoma 分泌蛋白（ASP）有关；鉴定出 25 个同源物，VAL-1 和 -2 也与寄生虫表面有关。VAL 蛋白的优势与Ancylostoma 和 Haemonchus ES 产物的报告相似。总的来说，这项研究表明 H. polygyrus 的分泌物与临床上重要的 GI 线虫的分泌物非常相似，证实了这种寄生虫作为寄生虫感染模型的价值。
