März Anne, Ackermann Katrin R, Malsch Daniéll, Bocklitz Thomas, Henkel Thomas, Popp Jürgen
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Jena, Germany.
J Biophotonics. 2009 Apr;2(4):232-42. doi: 10.1002/jbio.200810069.
In this contribution a new approach for quantitative measurements using surface-enhanced Raman spectroscopy (SERS) is presented. Combining the application of isotope-edited internal standard with the advantages of the liquid-liquid segmented-flow-based approach for flow-through SERS detection seems to be a promising means for quantitative SERS analysis. For the investigations discussed here a newly designed flow cell, tested for ideal mixing efficiency on the basis of grayscale-value measurements, is implemented. Measurements with the heteroaromatics nicotine and pyridine using their respective deuterated isotopomers as internal standards show that the integration of an isotopically labeled internal standard in the used liquid-liquid two-phase segmented flow leads to reproducible and comparable SERS spectra independent from the used colloid. With the implementation of an internal standard into the microfluidic device the influence of the properties of the colloid on the SERS activity can be compensated. Thus, the problem of a poor batch-to-batch reproducibility of the needed nanoparticle solutions is solved. To the best of our knowledge these are the first measurements combining the above mentioned concepts in order to correct for differences in the enhancement behaviour of the respective colloid.
本文介绍了一种使用表面增强拉曼光谱（SERS）进行定量测量的新方法。将同位素编辑内标的应用与基于液-液分段流的流通式SERS检测方法的优点相结合，似乎是定量SERS分析的一种有前途的手段。对于此处讨论的研究，采用了一种新设计的流通池，该流通池基于灰度值测量对理想混合效率进行了测试。使用各自的氘代同位素异构体作为内标对杂环芳烃尼古丁和吡啶进行测量，结果表明，在使用的液-液两相分段流中加入同位素标记的内标，可得到与所用胶体无关的可重复且可比的SERS光谱。通过在微流控装置中加入内标，可以补偿胶体性质对SERS活性的影响。因此，解决了所需纳米颗粒溶液批次间重现性差的问题。据我们所知，这些是首次将上述概念结合起来以校正各胶体增强行为差异的测量。
