Luts A, Uddman R, Grunditz T, Sundler F
Department of Medical Cell Research, Lund, Sweden.
J Auton Nerv Syst. 1990 Jul;30(3):179-91. doi: 10.1016/0165-1838(90)90249-i.
The distribution and origin of neuropeptide Y-, vasoactive intestinal peptide- and calcitonin gene-related peptide-containing nerve fibers and adrenergic (dopamine-beta-hydroxylase-containing) fibers in the rat larynx were studied by retrograde tracing and selective denervations in combination with immunocytochemistry. An injection of the retrograde tracer True Blue to the right vocal cord resulted in the appearance of labelled nerve cell bodies in the ipsi- and contralateral superior cervical and stellate ganglia, the thyroid ganglia, the jugular-nodose ganglionic complexes, in the ipsilateral trigeminal and dorsal root ganglia at levels C2 and C3 and in local tracheal ganglia. Judging from the number of labelled nerve cell bodies, the jugular-nodose ganglionic complexes, dorsal root ganglia and superior cervical ganglia provide the greater part of the vocal cord innervation. Most of the True Blue-labelled nerve cell bodies in the superior cervical and stellate ganglia contained neuropeptide Y. In the thyroid ganglia the majority of labelled nerve cell bodies contained vasoactive intestinal peptide. In the jugular-nodose ganglionic complex and the dorsal root ganglia the majority of the labelled nerve cell bodies stored calcitonin gene-related peptide. Retrograde tracing and denervation studies revealed that all noradrenaline- and the majority of neuropeptide Y-containing nerve fibers emanate from the superior cervical and stellate ganglia. A minor population of neuropeptide Y-containing nerve fibers originate in local tracheal ganglia. The vasoactive intestinal peptide-containing nerve fibers originate in the thyroid ganglion and local tracheal ganglia, whereas calcitonin gene-related peptide-containing nerve fibres emanate from the dorsal root ganglia (C2-C3), the trigeminal ganglia and the jugular-nodose ganglia.
通过逆行追踪和选择性去神经支配结合免疫细胞化学方法，研究了大鼠喉中含神经肽Y、血管活性肠肽和降钙素基因相关肽的神经纤维以及肾上腺素能（含多巴胺-β-羟化酶）纤维的分布和起源。将逆行示踪剂真蓝注射到右侧声带，导致同侧和对侧颈上神经节和星状神经节、甲状腺神经节、颈静脉-结状神经节复合体、C2和C3水平的同侧三叉神经节和背根神经节以及局部气管神经节中出现标记的神经细胞体。从标记的神经细胞体数量来看，颈静脉-结状神经节复合体、背根神经节和颈上神经节提供了大部分的声带神经支配。颈上神经节和星状神经节中大多数真蓝标记的神经细胞体含有神经肽Y。在甲状腺神经节中，大多数标记的神经细胞体含有血管活性肠肽。在颈静脉-结状神经节复合体和背根神经节中，大多数标记的神经细胞体储存降钙素基因相关肽。逆行追踪和去神经支配研究表明，所有去甲肾上腺素能和大多数含神经肽Y的神经纤维都起源于颈上神经节和星状神经节。一小部分含神经肽Y的神经纤维起源于局部气管神经节。含血管活性肠肽的神经纤维起源于甲状腺神经节和局部气管神经节，而含降钙素基因相关肽的神经纤维则起源于背根神经节（C2 - C3）、三叉神经节和颈静脉-结状神经节。
