Edvinsson L, Hara H, Uddman R
Department of Internal Medicine, University Hospital, Lund, Sweden.
J Cereb Blood Flow Metab. 1989 Apr;9(2):212-8. doi: 10.1038/jcbfm.1989.31.
The origin of nerve fibers to the rat middle cerebral artery was studied by retrograde tracing with the fluorescent tracer True Blue (TB) in combination with immunocytochemistry to known perivascular peptides. Application of TB to the middle cerebral artery labeled nerve cell bodies in the ipsilateral superior cervical ganglion, the otic ganglion, the sphenopalatine ganglion, the trigeminal ganglion, and the cervical dorsal root ganglion at level C2. A few labeled nerve cell bodies were seen in contralateral ganglia. Judging from the number and intensity of the labeling, the superior cervical ganglion and the trigeminal ganglion and dorsal root ganglion at level C2 contributed most to the innervation. A moderate number of nerve cell bodies were labeled in the sphenopalatine and otic ganglia. The TB-labeled nerve cell bodies were further examined for the presence of neuropeptides. For that purpose antibodies raised against neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP) were used. A considerable portion of the TB-labeled nerve cell bodies in the superior cervical ganglion contained NPY. About half of the labeled nerve cell bodies in the sphenopalatine and otic ganglia contained VIP. In the trigeminal ganglion and in the dorsal root ganglion at level C2, one-third of the TB-labeled nerve cell bodies were CGRP-immunoreactive, while only few nerve cell bodies contained SP. The study provides direct evidence for the origin of cerebrovascular peptidergic nerve fibers and demonstrates that not only ipsilateral but also contralateral ganglia contribute to the innervation of the cerebral circulation.
采用荧光示踪剂真蓝（TB）逆行追踪技术，并结合对已知血管周围肽的免疫细胞化学方法，研究了大鼠大脑中动脉神经纤维的起源。将TB应用于大脑中动脉后，同侧颈上神经节、耳神经节、蝶腭神经节、三叉神经节以及C2水平的颈背根神经节中的神经细胞体被标记。在对侧神经节中可见少数标记的神经细胞体。从标记的数量和强度判断，颈上神经节、三叉神经节和C2水平的背根神经节对神经支配的贡献最大。蝶腭神经节和耳神经节中有适量数量的神经细胞体被标记。对TB标记的神经细胞体进一步检测是否存在神经肽。为此，使用了针对神经肽Y（NPY）、血管活性肠多肽（VIP）、P物质（SP）和降钙素基因相关肽（CGRP）的抗体。颈上神经节中相当一部分TB标记的神经细胞体含有NPY。蝶腭神经节和耳神经节中约一半的标记神经细胞体含有VIP。在三叉神经节和C2水平的背根神经节中，三分之一的TB标记神经细胞体具有CGRP免疫反应性，而只有少数神经细胞体含有SP。该研究为脑血管肽能神经纤维的起源提供了直接证据，并表明不仅同侧神经节，对侧神经节也参与了脑循环的神经支配。
