Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Int J Food Microbiol. 2011 Sep 15;149(2):171-6. doi: 10.1016/j.ijfoodmicro.2011.06.002. Epub 2011 Jun 14.
Aspergillus section Nigri populations isolated from seven growing regions from Argentina were characterized by sequencing in order to identify species responsible for production of ochratoxin A (OTA) and fumonisins (FB(s)). Sequences of genes encoding calmodulin, β-tubulin, the second largest subunit of RNA polymerase II and translation elongation factor 1 alpha were analysed. The phylogenetic analysis showed the presence of six lineages: A. carbonarius, A. tubingensis, A. niger, A. japonicus, A. homomorphus and A. foetidus grouped in four major clusters. The molecular tools used allowed the identification for the first time of A. homomorphus from vineyards. OTA production confirmed the importance of A. carbonarius as the main ochratoxigenic species isolated and, to a variable degree, of A. niger and A. tubingensis, which were by far the most commonly occurring species on grapes in Argentina. The only strains able to produce OTA and fumonisins (B(2)-B(4)) belong to the A. niger cluster.
从阿根廷七个种植区分离的青霉 Nigri 种群通过测序进行了特征描述，以鉴定负责产生赭曲霉毒素 A（OTA）和伏马菌素（FB(s)）的物种。分析了编码钙调蛋白、β-微管蛋白、RNA 聚合酶 II 第二大亚基和翻译延伸因子 1α的基因序列。系统发育分析显示存在六个谱系：A. carbonarius、A. tubingensis、A. niger、A. japonicus、A. homomorphus 和 A. foetidus，分为四个主要簇。使用的分子工具首次鉴定出了来自葡萄园的 A. homomorphus。OTA 的产生证实了 A. carbonarius 作为主要产毒菌的重要性，以及程度不同的 A. niger 和 A. tubingensis，它们是迄今为止在阿根廷葡萄上最常见的物种。唯一能够产生 OTA 和伏马菌素（B(2)-B(4)）的菌株属于 A. niger 簇。
