World Class University (WCU) Program of Chemical Convergence for Energy & Environment (C2E2), School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Biomaterials. 2011 Oct;32(29):7217-25. doi: 10.1016/j.biomaterials.2011.06.020. Epub 2011 Jul 2.
The cellular response to conducting polymer (CP) nanospheres with similar physical properties was evaluated by in vitro cellular uptake and cytotoxicity in mouse macrophage RAW 264.7 and rat pheochromocytoma PC-12 cells. Four different CPs (polythiophene, poly(3,4-ethylenedioxythiophene), polyaniline, and polypyrrole) were deposited onto silica nanoparticles with a diameter of ca. 22 nm. Cellular uptake of silica/CP core/shell nanospheres in both cell lines was observed by transmission electron microscopy and they were internalized via phagocytosis and endocytosis. Cytotoxic effects were systemically assessed using live-cell microscopy, viability, oxidative stress, and lactate dehydrogenase assays. Silica/polythiophene core/shell nanospheres were the most toxic in both cell lines examined, because of the cellular effects of sulfur atoms. On the other hand, silica/polypyrrole core/shell nanospheres caused the lowest levels of toxicity in both cell lines. Furthermore, both rat and mouse cell viability was concentration-dependent with the nanospheres. These findings enhance nanotoxicological information regarding CP nanospheres when used with macrophage and neuronal cells, which may be useful in their application in bioelectronic and biomedical fields.
通过体外细胞摄取和小鼠巨噬细胞 RAW 264.7 和大鼠嗜铬细胞瘤 PC-12 细胞的细胞毒性评估了具有相似物理性质的导电聚合物 (CP) 纳米球的细胞反应。将四种不同的 CP（聚噻吩、聚（3,4-亚乙基二氧噻吩）、聚苯胺和聚吡咯）沉积到直径约为 22nm 的二氧化硅纳米颗粒上。通过透射电子显微镜观察到两种细胞系中二氧化硅/CP 核/壳纳米球的细胞摄取，它们通过吞噬作用和内吞作用被内化。使用活细胞显微镜、活力、氧化应激和乳酸脱氢酶测定系统地评估了细胞毒性作用。在两种被检测的细胞系中，二氧化硅/聚噻吩核/壳纳米球的毒性最大，因为硫原子对细胞有影响。另一方面，在两种细胞系中，二氧化硅/聚吡咯核/壳纳米球的毒性最低。此外，纳米球对大鼠和小鼠细胞活力的影响呈浓度依赖性。这些发现增强了关于 CP 纳米球在与巨噬细胞和神经元细胞一起使用时的纳米毒理学信息，这可能对其在生物电子学和生物医学领域的应用有用。
