Department of Physics, Annamalai University, Annamalainagar-608 002, TamilNadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):8-13. doi: 10.1016/j.saa.2011.04.082. Epub 2011 Jun 16.
The binding of rhodamine 6G and hydroxy propyl β-cyclodextrin (Hβ-CD) was investigated measuring fluorescence and absorption at pH 7.0. The solid inclusion complex of Rh6G and Hβ-CD has been studied by Ultraviolet (UV) spectroscopy, Fluorimetry, Fourier Transform Infrared (FTIR), (1)H Nuclear Magnetic Resonance ((1)HNMR) and in the Scanning Electron Microscope (SEM). Association constant K(g) and K(e) were determined by the enhancement of the fluorescence of rhodamine 6G in the presence of Hβ-CD. Fluorescence of Rh6G is generally enhanced, in complexes of Rh6G and β-Cyclodextrin in aqueous solutions. The free energy change for the ground state (ΔG(g)) and for the excited state (ΔG(e)) have also been determined. The experimental results indicated that the inclusion process is an exothermic and spontaneous.
在 pH 值为 7.0 时，通过测量荧光和吸收，研究了若丹明 6G 和羟丙基-β-环糊精（Hβ-CD）的结合情况。通过紫外（UV）光谱、荧光法、傅里叶变换红外（FTIR）、（1）H 核磁共振（（1）HNMR）和扫描电子显微镜（SEM）研究了 Rh6G 和 Hβ-CD 的固态包合复合物。通过在 Hβ-CD 存在下测定若丹明 6G 的荧光增强，确定了结合常数 K(g)和 K(e)。在水溶液中，Rh6G 与β-环糊精形成复合物时，其荧光通常会增强。还确定了基态（ΔG(g)）和激发态（ΔG(e)）的自由能变化。实验结果表明，包合过程是放热和自发的。
