• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鉴定水杨酰腙类毒力阻断化合物的细菌靶蛋白。

Identification of bacterial target proteins for the salicylidene acylhydrazide class of virulence-blocking compounds.

机构信息

Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.

出版信息

J Biol Chem. 2011 Aug 26;286(34):29922-31. doi: 10.1074/jbc.M111.233858. Epub 2011 Jul 1.

DOI:10.1074/jbc.M111.233858
PMID:21724850
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3191033/
Abstract

A class of anti-virulence compounds, the salicylidene acylhydrazides, has been widely reported to block the function of the type three secretion system of several Gram-negative pathogens by a previously unknown mechanism. In this work we provide the first identification of bacterial proteins that are targeted by this group of compounds. We provide evidence that their mode of action is likely to result from a synergistic effect arising from a perturbation of the function of several conserved proteins. We also examine the contribution of selected target proteins to the pathogenicity of Yersinia pseudotuberculosis and to expression of virulence genes in Escherichia coli O157.

摘要

一类抗毒化合物，即水杨醛酰腙，已被广泛报道通过一种先前未知的机制阻断几种革兰氏阴性病原体的 III 型分泌系统的功能。在这项工作中，我们首次鉴定了这组化合物的靶细菌蛋白。我们提供的证据表明，它们的作用模式可能是由于几种保守蛋白功能受到干扰而产生协同效应的结果。我们还研究了选定靶蛋白对假结核耶尔森氏菌致病性和大肠杆菌 O157 中毒力基因表达的贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/7ed9d2a2d558/zbc0401176120007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/14de67a67309/zbc0401176120002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/943d0392cc19/zbc0401176120003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/00ef5b3a5dc8/zbc0401176120004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/685efbf40644/zbc0401176120005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/7ed9d2a2d558/zbc0401176120007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/14de67a67309/zbc0401176120002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/943d0392cc19/zbc0401176120003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/00ef5b3a5dc8/zbc0401176120004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/685efbf40644/zbc0401176120005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c5/3191033/7ed9d2a2d558/zbc0401176120007.jpg

相似文献

1
Identification of bacterial target proteins for the salicylidene acylhydrazide class of virulence-blocking compounds.鉴定水杨酰腙类毒力阻断化合物的细菌靶蛋白。
J Biol Chem. 2011 Aug 26;286(34):29922-31. doi: 10.1074/jbc.M111.233858. Epub 2011 Jul 1.
2
Statistical molecular design of a focused salicylidene acylhydrazide library and multivariate QSAR of inhibition of type III secretion in the Gram-negative bacterium Yersinia.基于统计的水杨醛酰腙类化合物库的靶向设计及革兰氏阴性菌耶尔森氏菌 III 型分泌系统抑制的多元定量构效关系研究
Bioorg Med Chem. 2010 Apr 1;18(7):2686-703. doi: 10.1016/j.bmc.2010.02.022. Epub 2010 Feb 18.
3
Characterization of the effects of salicylidene acylhydrazide compounds on type III secretion in Escherichia coli O157:H7.水杨醛酰肼化合物对大肠杆菌O157:H7Ⅲ型分泌作用的表征
Infect Immun. 2009 Oct;77(10):4209-20. doi: 10.1128/IAI.00562-09. Epub 2009 Jul 27.
4
Structural characterisation of Tpx from Yersinia pseudotuberculosis reveals insights into the binding of salicylidene acylhydrazide compounds.从假结核耶尔森氏菌中 Tpx 的结构特征揭示了水杨醛酰腙类化合物结合的见解。
PLoS One. 2012;7(2):e32217. doi: 10.1371/journal.pone.0032217. Epub 2012 Feb 27.
5
Salicylidene acylhydrazides that affect type III protein secretion in Salmonella enterica serovar typhimurium.影响鼠伤寒沙门氏菌III型蛋白分泌的水杨醛酰肼类化合物。
Antimicrob Agents Chemother. 2007 Aug;51(8):2867-76. doi: 10.1128/AAC.00223-07. Epub 2007 Jun 4.
6
Small molecule inhibitors of LcrF, a Yersinia pseudotuberculosis transcription factor, attenuate virulence and limit infection in a murine pneumonia model.LcrF 是一种耶尔森氏菌属的转录因子，小分子抑制剂能减弱其毒力并限制其在小鼠肺炎模型中的感染。
Infect Immun. 2010 Nov;78(11):4683-90. doi: 10.1128/IAI.01305-09. Epub 2010 Sep 7.
7
IscR is essential for yersinia pseudotuberculosis type III secretion and virulence.IscR对于假结核耶尔森菌III型分泌和毒力至关重要。
PLoS Pathog. 2014 Jun 12;10(6):e1004194. doi: 10.1371/journal.ppat.1004194. eCollection 2014 Jun.
8
The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.丙酮酸-三羧酸循环节点：肠道病原体假结核耶尔森菌毒力控制的焦点
J Biol Chem. 2014 Oct 24;289(43):30114-32. doi: 10.1074/jbc.M114.581348. Epub 2014 Aug 27.
9
Genome Scale Analysis Reveals IscR Directly and Indirectly Regulates Virulence Factor Genes in Pathogenic .基因组规模分析揭示 IscR 直接和间接调节病原性. 中的毒力因子基因
mBio. 2021 Jun 29;12(3):e0063321. doi: 10.1128/mBio.00633-21. Epub 2021 Jun 1.
10
Crystal structures of WrbA, a spurious target of the salicylidene acylhydrazide inhibitors of type III secretion in Gram-negative pathogens, and verification of improved specificity of next-generation compounds.革兰氏阴性病原体 III 型分泌系统水杨醛酰腙抑制剂的伪靶标 WrbA 的晶体结构，以及下一代化合物改进特异性的验证。
Microbiology (Reading). 2022 Jul;168(7). doi: 10.1099/mic.0.001211.

引用本文的文献

1
Enterohaemorrhagic AdhE spirosome length correlates with enzymatic directionality and is perturbed by salicylidene acylhydrazides.肠出血性埃希氏菌黏附素螺旋体长度与酶促方向性相关，并受到水杨醛酰肼的干扰。
Open Biol. 2025 Jun;15(6):250041. doi: 10.1098/rsob.250041. Epub 2025 Jun 18.
2
Targeting bacterial pathogenesis by inhibiting virulence-associated Type III and Type IV secretion systems.通过抑制与毒力相关的 III 型和 IV 型分泌系统来靶向细菌发病机制。
Front Cell Infect Microbiol. 2023 Jan 10;12:1065561. doi: 10.3389/fcimb.2022.1065561. eCollection 2022.
3
Effects of the Quinone Oxidoreductase WrbA on Biofilm Formation and Oxidative Stress.

本文引用的文献

1
Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis.来自假结核耶尔森菌的硫醇过氧化物酶的表达、纯化、结晶及初步X射线衍射分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1606-9. doi: 10.1107/S1744309110039679. Epub 2010 Nov 25.
2
Synthesis of [4-(2-hydroxyphenyl)thiazol-2-yl]methanones as potential bioisosteres of salicylidene acylhydrazides.[4-(2-羟基苯基)噻唑-2-基]甲酮的合成作为水杨醛酰腙类生物等排体。
Molecules. 2010 Aug 31;15(9):6019-34. doi: 10.3390/molecules15096019.
3
Synthesis of 2-(2-aminopyrimidine)-2,2-difluoroethanols as potential bioisosters of salicylidene acylhydrazides.
醌氧化还原酶WrbA对生物膜形成和氧化应激的影响
Antioxidants (Basel). 2021 Jun 6;10(6):919. doi: 10.3390/antiox10060919.
4
Developing Cyclic Peptomers as Broad-Spectrum Type III Secretion System Inhibitors in Gram-Negative Bacteria.开发环状缩肽作为革兰氏阴性菌中广谱 III 型分泌系统抑制剂。
Antimicrob Agents Chemother. 2021 Jun 17;65(7):e0169020. doi: 10.1128/AAC.01690-20.
5
The Type III Secretion System: An Overview from Top to Bottom.III型分泌系统：从上到下的概述
Microorganisms. 2021 Feb 22;9(2):451. doi: 10.3390/microorganisms9020451.
6
Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.抑制细菌 III 型分泌系统（T3SS）的分子靶点和策略；直接结合 T3SS 成分的抑制剂。
Biomolecules. 2021 Feb 19;11(2):316. doi: 10.3390/biom11020316.
7
The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.利用纳米振动发现特定且有效的生物活性代谢物，刺激间充质干细胞的成骨分化。
Sci Adv. 2021 Feb 26;7(9). doi: 10.1126/sciadv.abb7921. Print 2021 Feb.
8
A Structure-Function-Inhibition Analysis of the Type III Secretion Needle Protein PscF.III 型分泌针蛋白 PscF 的结构-功能-抑制分析。
J Bacteriol. 2020 Aug 25;202(18). doi: 10.1128/JB.00055-20.
9
Identification of Benzyloxy Carbonimidoyl Dicyanide Derivatives as Novel Type III Secretion System Inhibitors High-Throughput Screening.鉴定苄氧基碳亚氨基二氰化物衍生物为新型III型分泌系统抑制剂的高通量筛选
Front Plant Sci. 2019 Sep 5;10:1059. doi: 10.3389/fpls.2019.01059. eCollection 2019.
10
On the road to structure-based development of anti-virulence therapeutics targeting the type III secretion system injectisome.在基于结构开发针对III型分泌系统注射体的抗毒力疗法的道路上。
Medchemcomm. 2019 Jun 20;10(8):1273-1289. doi: 10.1039/c9md00146h. eCollection 2019 Aug 1.
2-(2-氨基嘧啶)-2,2-二氟乙醇的合成及其作为水杨酰肼潜在生物等排体的研究。
Molecules. 2010 Jun 21;15(6):4423-38. doi: 10.3390/molecules15064423.
4
Candidate vaginal microbicides with activity against Chlamydia trachomatis and Neisseriagonorrhoeae.候选阴道杀菌剂对沙眼衣原体和淋病奈瑟菌有效。
Int J Antimicrob Agents. 2010 Aug;36(2):145-50. doi: 10.1016/j.ijantimicag.2010.03.018. Epub 2010 Jun 1.
5
Structure-based discovery of antibacterial drugs.基于结构的抗菌药物发现。
Nat Rev Microbiol. 2010 Jul;8(7):501-10. doi: 10.1038/nrmicro2349.
6
Statistical molecular design of balanced compound libraries for QSAR modeling.统计分子设计平衡化合物库用于 QSAR 建模。
Curr Med Chem. 2010;17(19):2001-16. doi: 10.2174/092986710791233661.
7
Thiol peroxidase protects Salmonella enterica from hydrogen peroxide stress in vitro and facilitates intracellular growth.硫氧还蛋白过氧化物酶可保护体外的沙门氏菌免受过氧化氢的应激，促进细胞内生长。
J Bacteriol. 2010 Jun;192(11):2929-32. doi: 10.1128/JB.01652-09. Epub 2010 Mar 19.
8
Statistical molecular design of a focused salicylidene acylhydrazide library and multivariate QSAR of inhibition of type III secretion in the Gram-negative bacterium Yersinia.基于统计的水杨醛酰腙类化合物库的靶向设计及革兰氏阴性菌耶尔森氏菌 III 型分泌系统抑制的多元定量构效关系研究
Bioorg Med Chem. 2010 Apr 1;18(7):2686-703. doi: 10.1016/j.bmc.2010.02.022. Epub 2010 Feb 18.
9
Anti-virulence strategies to combat bacteria-mediated disease.抗细菌毒力策略以对抗细菌介导的疾病。
Nat Rev Drug Discov. 2010 Feb;9(2):117-28. doi: 10.1038/nrd3013. Epub 2010 Jan 18.
10
Antivirulence drugs to target bacterial secretion systems.抗细菌分泌系统的抗病毒药物。
Curr Opin Microbiol. 2010 Feb;13(1):100-5. doi: 10.1016/j.mib.2009.12.003. Epub 2010 Jan 14.