The University of Queensland, Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, Health and Food Sciences Precinct, Coopers Plains, Australia.
J Agric Food Chem. 2011 Aug 10;59(15):8518-23. doi: 10.1021/jf201342t. Epub 2011 Jul 19.
Austral bracken, Pteridium esculentum , occurs widely in Australian grazing lands and contains both the known carcinogen ptaquiloside and its hydroxy analogue, ptesculentoside, with untested carcinogenic potential. Calves were fed a diet containing 19% P. esculentum that delivered 1.8 mg of ptaquiloside and 4.0 mg of ptesculentoside per kilogram of body weight (bw) per day to explore the carcass residue potential of these compounds. Concentrations of ptaquiloside and ptesculentoside in the liver, kidney, skeletal muscle, heart, and blood of these calves were determined as their respective elimination products, pterosin B and pterosin G, by HPLC-UV analysis. Plasma concentrations of up to 0.97 μg/mL ptaquiloside and 1.30 μg/mL ptesculentoside were found, but were shown to deplete to <10% of these values within 24 h of bracken consumption. Both glycosides were also detected in all tissues assayed, with ptesculentoside appearing to be more residual than ptaquiloside. Up to 0.42 and 0.32 μg/g ptesculentoside was present in skeletal muscle and liver, respectively, 15 days after bracken consumption ended. This detection of residual glycosides in tissues of cattle feeding on Austral bracken raises health concerns for consumers and warrants further investigation.
澳大拉西亚蕨，Pteridium esculentum ，广泛存在于澳大利亚放牧区，含有已知的致癌物质原蕨苷及其羟基类似物 ptesculentoside，具有未经测试的致癌潜力。小牛喂食含有 19% P. esculentum 的饮食，每天每公斤体重（BW）摄入 1.8 毫克原蕨苷和 4.0 毫克 ptesculentoside，以探索这些化合物在胴体残留中的潜力。通过 HPLC-UV 分析，确定这些小牛肝脏、肾脏、骨骼肌、心脏和血液中原蕨苷和 ptesculentoside 的浓度，作为其各自的消除产物 pterosin B 和 pterosin G。发现血浆中 ptaquiloside 和 ptesculentoside 的浓度高达 0.97 μg/mL 和 1.30 μg/mL，但在食用蕨菜后 24 小时内降至这些值的<10%。所有检测的组织中均检测到这两种糖苷，ptesculentoside 的残留量似乎比 ptaquiloside 更多。在食用蕨菜 15 天后，骨骼肌和肝脏中分别存在高达 0.42 和 0.32 μg/g 的 ptesculentoside。在食用澳大拉西亚蕨的牛的组织中检测到残留糖苷，这引起了消费者的健康担忧，需要进一步调查。
