• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用丹酚酸 B 调节口腔鳞状癌细胞的体外生长和血管生成潜力。

Modulation of growth and angiogenic potential of oral squamous carcinoma cells in vitro using salvianolic acid B.

机构信息

Department of General Dentistry, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011, China.

出版信息

BMC Complement Altern Med. 2011 Jul 5;11:54. doi: 10.1186/1472-6882-11-54.

DOI:10.1186/1472-6882-11-54
PMID:21726465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3158556/
Abstract

BACKGROUND

Our previous studies showed that Salvianolic acid B (Sal B) inhibited 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinogenesis in hamsters and such anti-cancer effects might be related to the inhibition of angiogenesis. This study was aimed to further investigate the anti-proliferative effect of Sal B on the most common type of oral cancer, oral squamous cell carcinoma (OSCC) and the possible mechanisms of action with respect to angiogenesis inhibition.

METHODS

Two well-characterized oral squamous cell carcinoma cell lines, CAL27 and SCC4, and premalignant leukoplakia cells were treated with different concentrations of Sal B. Cytotoxicity was assessed by MTT assay. cDNA microarray was utilized to evaluate the expression of 96 genes known to be involved in modulating the biological processes of angiogenesis. Real-time reverse transcription-polymerase chain reaction analysis was conducted to confirm the cDNA microarray data.

RESULTS

Sal B induced growth inhibition in OSCC cell lines but had limited effects on premalignant cells. A total of 17 genes showed a greater than 3-fold change when comparing Sal B treated OSCC cells to the control. Among these genes, HIF-1α, TNFα and MMP9 are specifically inhibited, expression of THBS2 was up-regulated.

CONCLUSIONS

Sal B has inhibitory effect on OSCC cell growth. The antitumor effect can be attributed to anti-angiogenic potential induced by a decreased expression of some key regulator genes of angiogenesis. Sal B may be a promising modality for treating oral squamous cell carcinoma.

摘要

背景

我们之前的研究表明，丹酚酸 B（Sal B）抑制了 7,12-二甲基苯并[a]蒽（DMBA）诱导的仓鼠口腔致癌作用，这种抗癌作用可能与抑制血管生成有关。本研究旨在进一步研究 Sal B 对最常见的口腔癌——口腔鳞状细胞癌（OSCC）的抗增殖作用及其对血管生成抑制的可能作用机制。

方法

用不同浓度的 Sal B 处理两种经过充分鉴定的口腔鳞状细胞癌细胞系 CAL27 和 SCC4 以及癌前白斑细胞。通过 MTT 法评估细胞毒性。利用 cDNA 微阵列评估已知参与调节血管生成生物学过程的 96 个基因的表达。通过实时逆转录聚合酶链反应分析来验证 cDNA 微阵列数据。

结果

Sal B 诱导 OSCC 细胞系生长抑制，但对癌前细胞的作用有限。与对照组相比，OSCC 细胞中 17 个基因的表达变化超过 3 倍。在这些基因中，HIF-1α、TNFα 和 MMP9 被特异性抑制，THBS2 的表达上调。

结论

Sal B 对 OSCC 细胞生长具有抑制作用。抗肿瘤作用归因于某些关键血管生成调节基因表达下调引起的抗血管生成潜力。Sal B 可能是治疗口腔鳞状细胞癌的一种有前途的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/958f279e77bc/1472-6882-11-54-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/5eb186f9798f/1472-6882-11-54-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/aba56804f12d/1472-6882-11-54-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/958f279e77bc/1472-6882-11-54-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/5eb186f9798f/1472-6882-11-54-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/aba56804f12d/1472-6882-11-54-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef6/3158556/958f279e77bc/1472-6882-11-54-3.jpg

相似文献

1
Modulation of growth and angiogenic potential of oral squamous carcinoma cells in vitro using salvianolic acid B.利用丹酚酸 B 调节口腔鳞状癌细胞的体外生长和血管生成潜力。
BMC Complement Altern Med. 2011 Jul 5;11:54. doi: 10.1186/1472-6882-11-54.
2
Salvianolic acid B inhibits glycolysis in oral squamous cell carcinoma via targeting PI3K/AKT/HIF-1α signaling pathway.丹酚酸 B 通过靶向 PI3K/AKT/HIF-1α 信号通路抑制口腔鳞状细胞癌糖酵解。
Cell Death Dis. 2018 May 22;9(6):599. doi: 10.1038/s41419-018-0623-9.
3
The preventive effect of salvianolic acid B on malignant transformation of DMBA-induced oral premalignant lesion in hamsters.丹酚酸B对二甲基苯并蒽诱导的仓鼠口腔癌前病变恶变的预防作用。
Carcinogenesis. 2006 Apr;27(4):826-32. doi: 10.1093/carcin/bgi271. Epub 2005 Nov 14.
4
Salvianolic acid B inhibits growth of head and neck squamous cell carcinoma in vitro and in vivo via cyclooxygenase-2 and apoptotic pathways.丹酚酸B通过环氧合酶-2和凋亡途径在体外和体内抑制头颈部鳞状细胞癌的生长。
Int J Cancer. 2009 May 1;124(9):2200-9. doi: 10.1002/ijc.24160.
5
Crude extract of Salvia miltiorrhiza and salvianolic acid B enhance in vitro angiogenesis in murine SVR endothelial cell line.丹参粗提物和丹酚酸B增强小鼠SVR内皮细胞系的体外血管生成。
Planta Med. 2003 Jan;69(1):26-32. doi: 10.1055/s-2003-37034.
6
Anti-cancer effect of genistein in oral squamous cell carcinoma with respect to angiogenesis and in vitro invasion.金雀异黄素对口腔鳞状细胞癌血管生成及体外侵袭的抗癌作用。
Cancer Sci. 2003 Feb;94(2):215-20. doi: 10.1111/j.1349-7006.2003.tb01422.x.
7
The inhibitory effect of salvianolic acid B on TGF-β1-induced proliferation and differentiation in lung fibroblasts.丹酚酸B对转化生长因子-β1诱导的肺成纤维细胞增殖和分化的抑制作用。
Exp Lung Res. 2014 Apr;40(4):172-85. doi: 10.3109/01902148.2014.895070. Epub 2014 Mar 26.
8
EMODIN DOWNREGULATES CELL PROLIFERATION MARKERS DURING DMBA INDUCED ORAL CARCINOGENESIS IN GOLDEN SYRIAN HAMSTERS.在二甲基苯并蒽诱导的叙利亚金黄地鼠口腔致癌过程中，大黄素下调细胞增殖标志物。
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):83-91. doi: 10.21010/ajtcam.v14i2.10. eCollection 2017.
9
Transformation of salvianolic acid B to salvianolic acid a in aqueous solution and the in vitro liver protective effect of the main products.丹酚酸B在水溶液中向丹酚酸A的转化及其主要产物的体外肝脏保护作用。
J Food Sci. 2014 Apr;79(4):C499-504. doi: 10.1111/1750-3841.12415. Epub 2014 Apr 1.
10
Anti-cell proliferative and anti-angiogenic potential of andrographolide during 7,12- dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis.穿心莲内酯在7,12-二甲基苯并(a)蒽诱导的仓鼠颊囊癌变过程中的抗细胞增殖和抗血管生成潜力。
Asian Pac J Cancer Prev. 2013;14(10):6001-5. doi: 10.7314/apjcp.2013.14.10.6001.

引用本文的文献

1
Salvianolic acid B from bunge: A potential antitumor agent.来自丹参的丹酚酸B：一种潜在的抗肿瘤剂。
Front Pharmacol. 2022 Oct 25;13:1042745. doi: 10.3389/fphar.2022.1042745. eCollection 2022.
2
Possible applications of salvianolic acid B against different cancers.丹酚酸B在不同癌症治疗中的潜在应用。
Explor Target Antitumor Ther. 2020;1(4):218-238. doi: 10.37349/etat.2020.00014. Epub 2020 Aug 31.
3
Pharmacological Effects of Salvianolic Acid B Against Oxidative Damage.丹酚酸B对氧化损伤的药理作用

本文引用的文献

1
Combination effects of salvianolic acid B with low-dose celecoxib on inhibition of head and neck squamous cell carcinoma growth in vitro and in vivo.丹参多酚酸 B 联合小剂量塞来昔布对体外及体内头颈部鳞癌细胞生长的抑制作用
Cancer Prev Res (Phila). 2010 Jun;3(6):787-96. doi: 10.1158/1940-6207.CAPR-09-0243. Epub 2010 May 25.
2
Anti-inflammatory activity of salvianolic acid B in microglia contributes to its neuroprotective effect.丹酚酸 B 通过小胶质细胞发挥抗炎作用，从而起到神经保护作用。
Neurochem Res. 2010 Jul;35(7):1029-37. doi: 10.1007/s11064-010-0151-1. Epub 2010 Mar 18.
3
Implanted microvessels progress through distinct neovascularization phenotypes.
Front Pharmacol. 2020 Nov 11;11:572373. doi: 10.3389/fphar.2020.572373. eCollection 2020.
4
Network pharmacology exploration reveals a common mechanism in the treatment of cardio-cerebrovascular disease with Salvia miltiorrhiza Burge. and Carthamus tinctorius L.网络药理学探索揭示丹参和红花治疗心脑血管疾病的共同作用机制。
BMC Complement Med Ther. 2020 Nov 19;20(1):351. doi: 10.1186/s12906-020-03026-y.
5
Salvianolic Acid B Slows the Progression of Breast Cancer Cell Growth via Enhancement of Apoptosis and Reduction of Oxidative Stress, Inflammation, and Angiogenesis.丹酚酸 B 通过增强细胞凋亡、减少氧化应激、炎症和血管生成来减缓乳腺癌细胞的生长速度。
Int J Mol Sci. 2019 Nov 12;20(22):5653. doi: 10.3390/ijms20225653.
6
Salvianolic acid A & B: potential cytotoxic polyphenols in battle against cancer via targeting multiple signaling pathways.丹酚酸 A、B：通过靶向多种信号通路，用于抗癌的潜在细胞毒性多酚。
Int J Biol Sci. 2019 Aug 19;15(10):2256-2264. doi: 10.7150/ijbs.37467. eCollection 2019.
7
Salvianolic Acids: Potential Source of Natural Drugs for the Treatment of Fibrosis Disease and Cancer.丹酚酸：治疗纤维化疾病和癌症的天然药物潜在来源。
Front Pharmacol. 2019 Feb 20;10:97. doi: 10.3389/fphar.2019.00097. eCollection 2019.
8
Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation.丹酚酸B通过p38介导的活性氧生成抑制骨肉瘤细胞增殖并诱导其凋亡。
Oncol Lett. 2018 Feb;15(2):2679-2685. doi: 10.3892/ol.2017.7609. Epub 2017 Dec 13.
9
Danshen extract circumvents drug resistance and represses cell growth in human oral cancer cells.丹参提取物可规避人口腔癌细胞的耐药性并抑制其细胞生长。
BMC Complement Altern Med. 2017 Dec 29;17(1):555. doi: 10.1186/s12906-017-2063-y.
10
RBP4 and THBS2 are serum biomarkers for diagnosis of colorectal cancer.视黄醇结合蛋白4（RBP4）和血小板反应蛋白2（THBS2）是用于诊断结直肠癌的血清生物标志物。
Oncotarget. 2017 Sep 21;8(54):92254-92264. doi: 10.18632/oncotarget.21173. eCollection 2017 Nov 3.
植入的微血管经历不同的血管新生表型。
Microvasc Res. 2010 Jan;79(1):10-20. doi: 10.1016/j.mvr.2009.10.001. Epub 2009 Oct 13.
4
Effects of salvianolic acids on oxidative stress and hepatic fibrosis in rats.丹酚酸对大鼠氧化应激和肝纤维化的影响。
Toxicol Appl Pharmacol. 2010 Jan 15;242(2):155-64. doi: 10.1016/j.taap.2009.10.002. Epub 2009 Oct 12.
5
Salvianolic acid B inhibits growth of head and neck squamous cell carcinoma in vitro and in vivo via cyclooxygenase-2 and apoptotic pathways.丹酚酸B通过环氧合酶-2和凋亡途径在体外和体内抑制头颈部鳞状细胞癌的生长。
Int J Cancer. 2009 May 1;124(9):2200-9. doi: 10.1002/ijc.24160.
6
Comparison of cardioprotective effects of salvianolic acid B and benazepril on large myocardial infarction in rats.丹酚酸B与苯那普利对大鼠大面积心肌梗死心脏保护作用的比较
Pharmacol Rep. 2008 May-Jun;60(3):369-81.
7
Over-expression of 14-3-3sigma in budding colorectal cancer cells modulates cell migration in the presence of tenascin-C.在萌芽状态的结肠癌细胞中，14-3-3σ的过表达在有腱生蛋白-C存在的情况下调节细胞迁移。
Oncol Rep. 2007 Dec;18(6):1451-6.
8
Osteopontin gene expression determines spontaneous metastatic performance of orthotopic human breast cancer xenografts.骨桥蛋白基因表达决定原位人乳腺癌异种移植瘤的自发转移性能。
Am J Pathol. 2007 Aug;171(2):682-92. doi: 10.2353/ajpath.2007.070232. Epub 2007 Jul 9.
9
Salvianolic acid B improves motor function after cerebral ischemia in rats.丹酚酸B可改善大鼠脑缺血后的运动功能。
Behav Pharmacol. 2006 Sep;17(5-6):493-8. doi: 10.1097/00008877-200609000-00015.
10
Salvianolic acid B attenuates MMP-2 and MMP-9 expression in vivo in apolipoprotein-E-deficient mouse aorta and in vitro in LPS-treated human aortic smooth muscle cells.丹酚酸B在载脂蛋白E缺陷小鼠主动脉中可在体内减弱基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）的表达，在脂多糖处理的人主动脉平滑肌细胞中可在体外减弱其表达。
J Cell Biochem. 2007 Feb 1;100(2):372-84. doi: 10.1002/jcb.21042.