Departamento de Química Analítica y Alimentaria, Área de Química Analítica, Facultad de Química, Universidad de Vigo, Campus As Lagoas-Marcosende s/n, 36310 Vigo, Spain.
Talanta. 2011 Aug 15;85(2):1100-4. doi: 10.1016/j.talanta.2011.05.032. Epub 2011 May 27.
A miniaturized methodology for the determination of phosphate in waters has been developed by combining directly suspended droplet microextraction (DSDME) with microvolume spectrophotometry. The method is based on the extraction of the ion pair formed between 12-molybdophosphate and malachite green onto a microdrop of methyl isobutyl ketone and subsequent spectrophotometric determination with no dilution. An enrichment factor of 325 was obtained after 7.5 min of microextraction. The detection limit was 6.1 nM phosphate and the repeatability, expressed as relative standard deviation, was 2.7% (n=6). The method was successfully applied to the determination of dissolved reactive phosphorus in different freshwater samples.
一种将直接悬浮液滴微萃取（DSDME）与微量分光光度法相结合的测定水中磷酸盐的微型化方法已经开发出来。该方法基于将 12-钼磷酸盐和孔雀绿之间形成的离子对萃取到甲基异丁基酮的微滴中，并且无需稀释即可进行分光光度法测定。在微萃取 7.5 分钟后，可获得 325 的富集因子。检测限为 6.1 nM 磷酸盐，重复性（表示为相对标准偏差）为 2.7%（n=6）。该方法成功应用于不同淡水样品中溶解活性磷的测定。
