Departamento de Química Analítica y Alimentaria, Área de Química Analítica, Facultad de Química, Universidad de Vigo, Campus As Lagoas-Marcosende s/n, 36310 Vigo, Spain.
Talanta. 2012 Jan 30;89:217-22. doi: 10.1016/j.talanta.2011.11.084. Epub 2011 Dec 9.
A rapid and simple screening method for polycyclic aromatic hydrocarbons (PAHs) in water samples is proposed. The method is based on the combination of a miniaturized sample preparation approach, namely, directly suspended droplet microextraction (DSDME), and microvolume fluorospectrometry. Benzo[a]pyrene (BaP) was used as the model compound for screening purposes. Under optimal conditions, a detection limit of 0.024 μg L(-1) and an enrichment factor of 159 were obtained for BaP in 5 min. The repeatability, expressed as relative standard deviation (RSD), was 4.9% (n=8). The unreliability region of the screening method was 0.54-0.67 μg L(-1), by using a cut-off value of 0.6 μg L(-1) of BaP. Finally, the proposed method was applied to the in situ achievement of the binary "yes/no" response for PAHs in different water samples and recovery studies were performed at three different levels, with BaP recoveries in the range of 93-104%.
提出了一种快速、简单的水样中多环芳烃（PAHs）的筛选方法。该方法基于微型化样品制备方法（即直接悬浮液滴微萃取（DSDME））和微量荧光光谱法的结合。苯并[a]芘（BaP）被用作筛选目的的模型化合物。在最佳条件下，在 5 分钟内可获得 BaP 的检测限为 0.024μg/L，富集因子为 159。BaP 的相对标准偏差（RSD）重复性为 4.9%（n=8）。通过使用 0.6μg/L 的 BaP 作为截断值，筛选方法的不可靠区域为 0.54-0.67μg/L。最后，该方法应用于不同水样中 PAHs 的原位实现二元“是/否”响应，并在三个不同水平上进行了回收率研究，BaP 的回收率范围为 93-104%。
