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影响酵母转座的宿主基因：一种稀有tRNA的丰度调节Ty1转座频率。

Host genes that influence transposition in yeast: the abundance of a rare tRNA regulates Ty1 transposition frequency.

作者信息

Xu H, Boeke J D

机构信息

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

出版信息

Proc Natl Acad Sci U S A. 1990 Nov;87(21):8360-4. doi: 10.1073/pnas.87.21.8360.

DOI:10.1073/pnas.87.21.8360
PMID:2172984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC54955/
Abstract

Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single copy tRNA(CCU Arg) gene as one of the genes and reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNA(CCU Arg) in yeast cells.

摘要

在高拷贝数载体中对酵母基因组文库进行遗传筛选，确定了通常为单拷贝的tRNA（CCU Arg）基因是其中一个基因，并且该基因过表达时会降低Ty1转座频率。对Ty1编码蛋白的免疫印迹分析表明，tRNA基因的拷贝数与TYB蛋白的产生呈负相关。因此，Ty1转座频率显然受酵母细胞中tRNA（CCU Arg）水平的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/0592d97c060f/pnas01046-0186-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/ee94a0eb4122/pnas01046-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/0d8b17fe3b09/pnas01046-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/6514d1854f0a/pnas01046-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/0592d97c060f/pnas01046-0186-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/ee94a0eb4122/pnas01046-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/0d8b17fe3b09/pnas01046-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/6514d1854f0a/pnas01046-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fe9/54955/0592d97c060f/pnas01046-0186-c.jpg

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本文引用的文献

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