Zhang Kui-Hua, Yu Qiao-Zhen, Mo Xiu-Mei
College of Materials and Textile Engineering, Jiaxing University, Zhejiang 314001, China; E-Mail:
Int J Mol Sci. 2011;12(4):2187-99. doi: 10.3390/ijms12042187. Epub 2011 Mar 30.
The native extracellular matrix (ECM) is composed of a cross-linked porous network of multifibril collagens and glycosaminoglycans. Nanofibrous scaffolds of silk fibroin (SF) and hydroxybutyl chitosan (HBC) blends were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic acid (TFA) as solvents to biomimic the native ECM via electrospinning. Scanning electronic microscope (SEM) showed that relatively uniform nanofibers could be obtained when 12% SF was blended with 6% HBC at the weight ratio of 50:50. Meanwhile, the average nanofibrous diameter increased when the content of HBC in SF/HBC blends was raised from 20% to 100%. Fourier transform infrared spectra (FTIR) and (13)C nuclear magnetic resonance (NMR) showed SF and HBC molecules existed in hydrogen bonding interactions but HBC did not induce conformation of SF transforming from random coil form to β-sheet structure. X-ray diffraction (XRD) confirmed the different structure of SF/HBC blended nanofibers from both SF and HBC. Thermogravimetry-Differential thermogravimetry (TG-DTG) results demonstrated that the thermal stability of SF/HBC blend nanofibrous scaffolds was improved. The results indicated that the rearrangement of HBC and SF molecular chain formed a new structure due to stronger hydrogen bonding between SF and HBC. These electrospun SF/HBC blended nanofibers may provide an ideal tissue engineering scaffold and wound dressing.
天然细胞外基质（ECM）由多纤维胶原蛋白和糖胺聚糖的交联多孔网络组成。以1,1,1,3,3,3-六氟-2-丙醇（HFIP）和三氟乙酸（TFA）作为溶剂，通过静电纺丝制备了丝素蛋白（SF）和羟丁基壳聚糖（HBC）共混的纳米纤维支架，以仿生天然ECM。扫描电子显微镜（SEM）显示，当12%的SF与6%的HBC以50:50的重量比混合时，可以获得相对均匀的纳米纤维。同时，当SF/HBC共混物中HBC的含量从20%提高到100%时，纳米纤维的平均直径增加。傅里叶变换红外光谱（FTIR）和碳-13核磁共振（NMR）表明，SF和HBC分子以氢键相互作用存在，但HBC并未诱导SF的构象从无规卷曲形式转变为β-折叠结构。X射线衍射（XRD）证实了SF/HBC共混纳米纤维与SF和HBC的结构不同。热重-微商热重（TG-DTG）结果表明，SF/HBC共混纳米纤维支架的热稳定性得到了提高。结果表明，由于SF和HBC之间较强的氢键作用，HBC和SF分子链的重排形成了一种新结构。这些静电纺丝的SF/HBC共混纳米纤维可能提供一种理想的组织工程支架和伤口敷料。
