Division of Molecular Entomology, Department of Zoology, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India.
Parasitol Res. 2012 Jan;110(1):381-8. doi: 10.1007/s00436-011-2502-5. Epub 2011 Jul 6.
Integrated vector control is an effective and essential part of any successful vector control program. Increasing insecticide resistance requires strategies to prolong the use of highly effective vector control compounds. Synergistic activity between current effective pesticides is a powerful tool and is one such strategy. In the current study, Cyt1A from Bacillus thurigiensis subsp. israelensis and Bacillus sphaericus B101H5aH5b Bs were evaluated for the synergistic activity against B. sphaericus-resistant strains of Anopheles stephensi. The combinations of Cyt1A and B. sphaericus were found to act synergistically and were greatly enhanced at different ratios. A ratio of 1:4 of Cyt1A and Bs was 13,200-fold more toxic at LC(90) against the Bs-resistant strains of A. stephensi than was Bs alone and this high level of activity resulted from synergism between Cyt1A toxin and Bs. Our results therefore suggest that Cyt1A may enhance toxicity by facilitating the binding or insertion of the binary toxins to the microvillar membrane.
综合虫害治理是任何成功的虫害治理计划的有效且必要的组成部分。杀虫剂抗性的增加需要延长高效虫害治理化合物使用的策略。当前有效的杀虫剂之间的协同作用是一种强大的工具，是此类策略之一。在目前的研究中，评估了苏云金芽孢杆菌亚种以色列亚种和球形芽孢杆菌 B101H5aH5b Bs 的 Cyt1A 对致倦库蚊抗球形芽孢杆菌菌株的协同活性。发现 Cyt1A 和 Bs 的组合具有协同作用，并以不同的比例大大增强。Cyt1A 和 Bs 的比例为 1:4 时，对致倦库蚊抗球形芽孢杆菌菌株的 LC(90) 值比单独使用 Bs 高 13200 倍，这种高活性是 Cyt1A 毒素和 Bs 之间协同作用的结果。因此，我们的研究结果表明，Cyt1A 可能通过促进二元毒素与微绒毛膜的结合或插入来增强毒性。
