College of Pharmacy, SahmYook University, Seoul 139-742, Republic of Korea.
J Inflamm (Lond). 2011 Jul 7;8(1):16. doi: 10.1186/1476-9255-8-16.
Arctiin, isolated from Forsythia suspensa has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects in vitro. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages.
We investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes.
Arctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE2, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-κB in macrophages was inhibited by arctiin.
Taken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.
从连翘中分离得到的牛蒡子苷已被报道具有抗炎、抗氧化、抗菌和抗病毒的体外作用。然而，关于其对免疫活性的影响的研究还很少。本研究旨在探讨牛蒡子苷对 LPS 诱导的巨噬细胞的抗炎潜力及其可能的机制。
我们通过 RT-PCR 和 Western blot 分析研究了促炎细胞因子的 mRNA 和蛋白水平，然后通过 FACS 分析研究了表面分子的变化。
牛蒡子苷呈剂量依赖性降低 NO 和促炎细胞因子（如 IL-1β、IL-6、TNF-α 和 PGE2）的产生，通过 RT-PCR 和 Western blot 分析分别降低基因和蛋白水平。牛蒡子苷还抑制共刺激分子如 B7-1 和 B7-2 的表达。此外，牛蒡子苷还抑制了巨噬细胞中核转录因子 NF-κB 的激活。
综上所述，这些结果为牛蒡子苷在炎症性疾病中的生物活性提供了证据，并表明牛蒡子苷可能通过抑制 NF-kB 的失活来抑制促炎介质发挥抗炎作用。
