Graduate Programme in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Chulalongkorn University, Bangkok, Thailand.
Epidemiol Infect. 2012 Mar;140(3):539-41. doi: 10.1017/S0950268811001294. Epub 2011 Jul 7.
Seventy-five clinical isolates of Pseudomonas aeruginosa collected in a tertiary teaching hospital in Thailand were investigated for susceptibility to antimicrobials including imipenem. Metallo-β-lactamase (MBL) enzymes were detected by E-test MBL assay and PCR; class 1 integron genes were also detected by PCR. Strains positive for bla(IMP) and bla(VIM) genes were further characterized by DNA sequencing and examined for clonality by pulsed-field gel electrophoresis. High rates of resistance to anti-pseudomonal agents were found. MBL enzymes were found in 13 (17·3%) strains and 24 (32%) carried class 1 integron genes. Twelve of the latter strains harboured the bla(IMP-14) gene and one strain the bla(VIM-2) gene. All of the IMP-14 strains were identical or closely related suggesting clonal dissemination of these genes.
在泰国的一家三级教学医院收集的 75 株铜绿假单胞菌临床分离株被检测对包括亚胺培南在内的抗菌药物的敏感性。金属β-内酰胺酶（MBL）酶通过 E 试验 MBL 测定和 PCR 检测；通过 PCR 还检测了 class 1 整合子基因。对 bla(IMP)和 bla(VIM)基因阳性的菌株进行了 DNA 测序进一步鉴定，并通过脉冲场凝胶电泳检查了其克隆性。发现对抗假单胞菌药物的耐药率很高。在 13 株（17.3%）菌株中发现了 MBL 酶，24 株（32%）携带 class 1 整合子基因。后者中有 12 株菌株携带 bla(IMP-14)基因，1 株菌株携带 bla(VIM-2)基因。所有的 IMP-14 菌株都是相同或密切相关的，表明这些基因的克隆传播。
