Khuntayaporn Piyatip, Yamprayoonswat Wariya, Yasawong Montri, Chomnawang Mullika Traidej
Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok, Thailand.
Infect Chemother. 2019 Jun;51(2):107-118. doi: 10.3947/ic.2019.51.2.107.
is considered as one of the most emerging threats in this century. Serious infections caused by this pathogen are often treated by carbapenems which are the last resource of antibiotics. Metallo-beta-lactamases (MBLs) production is one of the most important carbepenem resistance mechanisms and is usually related with nosocomial infections caused by . This study was aimed to determine the prevalence of MBL genes and distribution pattern of MBLs producing strains in Thailand.
Specific primers were designed to detect MBL genes including IMP-, VIM-, and NDM-type MBL genes. Multilocus sequence typing method was used to determine the dissemination pattern of carbapenem-resistance among multidrug-resistant (CR-MDR) .
A total of 153 clinical isolates were characterized as CR-MDR. Among those, 31 clinical isolates (20.3%) presented metallo-beta-lactamase genes which could be divided into VIM-type (14 strains) and IMP-type (17 strains). , , , and genes were detected. Moreover, a novel IMP-type MBL, was discovered and it was demonstrated to be the unique group of MBLs in Thailand. It was of interest that ST235 was the major ST type in Thailand followed by ST964 and ST111 and ST235 was detected in both MBL harboring and non-MBL harboring strains.
This study reported the dissemination of MBL gene including novel MBL, . This study was also demonstrated major ST of which was ST235, followed by ST964 and ST111. Moreover, it is also the first report on many STs in Thailand: ST273, ST292, ST621, ST1584, and ST1816 which emphasized the dissemination trait difference of MBLs harboring in Thailand.
在本世纪，它被视为最新出现的威胁之一。由这种病原体引起的严重感染通常用碳青霉烯类药物治疗，而碳青霉烯类药物是抗生素的最后一道防线。金属β-内酰胺酶（MBLs）的产生是最重要的碳青霉烯耐药机制之一，通常与由该病原体引起的医院感染有关。本研究旨在确定泰国产MBL基因的流行情况以及产MBL菌株的分布模式。
设计特异性引物以检测包括IMP型、VIM型和NDM型MBL基因在内的MBL基因。采用多位点序列分型方法来确定耐碳青霉烯类多重耐药（CR-MDR）菌株中碳青霉烯耐药的传播模式。
总共153株临床分离株被鉴定为CR-MDR。其中，31株临床分离株（20.3%）呈现金属β-内酰胺酶基因，可分为VIM型（14株）和IMP型（17株）。检测到了、、和基因。此外，还发现了一种新型的IMP型MBL，并且证明它是泰国MBL的独特类型。有趣的是，ST235是泰国的主要ST型，其次是ST964、ST111，并且在携带MBL和不携带MBL的菌株中均检测到了ST235。
本研究报告了包括新型MBL在内的MBL基因的传播情况。本研究还证明了该病原体的主要ST型是ST235，其次是ST964和ST111。此外，这也是泰国关于许多该病原体ST型（ST273、ST292、ST621、ST1584和ST1816）的首次报告，强调了泰国携带MBL病原体的传播特征差异。
