Poulsen D J, Burton C R, O'Brian J J, Rabin S J, Keeler C L
Department of Animal Science and Agricultural Biochemistry, College of Agricultural Sciences, University of Delaware, Newark 19717-1303.
Virus Genes. 1991 Oct;5(4):335-47. doi: 10.1007/BF00271532.
The infectious laryngotracheitis virus (ILTV) homologue of the herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene was identified by PCR amplification of genomic ILTV DNA. A 488-bp amplified DNA fragment was used to identify and clone two adjacent PstI fragments from genomic ILTV DNA. Sequence analysis of the region surrounding the amplified fragment identified a 2619-bp open reading frame that has 39% homology with both the nucleotide and amino-acid sequences of the HSV-1 gB gene. Northern blot analysis using a portion of the open reading frame as a probe identified a 2.7-kb RNA transcript in ILTV-infected chicken embryo liver cells. Analysis of the predicted amino acid sequence of the ILTV protein indicated that it shares structural features with the gB glycoproteins of other herpesviruses.
通过对传染性喉气管炎病毒（ILTV）基因组DNA进行PCR扩增，鉴定出了与单纯疱疹病毒1型（HSV-1）糖蛋白B（gB）基因同源的ILTV基因。用一个488 bp的扩增DNA片段从ILTV基因组DNA中鉴定并克隆出两个相邻的PstI片段。对扩增片段周围区域的序列分析确定了一个2619 bp的开放阅读框，该阅读框与HSV-1 gB基因的核苷酸和氨基酸序列均具有39%的同源性。用开放阅读框的一部分作为探针进行Northern印迹分析，在感染ILTV的鸡胚肝细胞中鉴定出一个2.7 kb的RNA转录本。对ILTV蛋白预测氨基酸序列的分析表明，它与其他疱疹病毒的gB糖蛋白具有共同的结构特征。
