Department of Pharmacology, Instituto de Ciencias Biologicas, Antonio Carlos, Brazil.
Eur J Dermatol. 2011 Sep-Oct;21(5):722-30. doi: 10.1684/ejd.2011.1466.
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of skin healing activity by the group of proteinases known as P1G10. By using a hairless mouse model, we compared the healing effect following topical application of various concentrations of P1G10. The data confirm that healing actions take place between 0.1 and 1%, without adverse local irritation or systemic toxicological action after a prolonged period of use. The wound healing effect is unaltered when P1G10 is previously inhibited with iodoacetamide. The low permeation of the hydrosoluble formulation Polawax(®) supports the maintenance of the drug at the site of application. These results extend the healing properties of these groups of enzymes in situations of dermatological trauma and open the way to future clinical applications.
先前的研究表明，来自 C. candamarcensis 乳胶的蛋白酶对成纤维细胞和上皮细胞具有有丝分裂原的作用，随后的一份报告显示它们对胃溃疡具有保护、血管生成和伤口愈合作用。在这项研究中，我们通过一组称为 P1G10 的蛋白酶提供了皮肤愈合活性的证据。通过使用无毛小鼠模型，我们比较了外用不同浓度的 P1G10 后的愈合效果。数据证实，在长时间使用后，愈合作用发生在 0.1%至 1%之间，没有局部刺激或全身毒性作用。在用碘乙酰胺预先抑制 P1G10 后，其愈合作用保持不变。水溶性配方 Polawax(®) 的低渗透性支持药物在应用部位的维持。这些结果扩展了这些酶类在皮肤创伤情况下的愈合特性，并为未来的临床应用开辟了道路。
