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禽逆转录病毒DNA在体外的高效自身整合

Efficient autointegration of avian retrovirus DNA in vitro.

作者信息

Lee Y M, Coffin J M

机构信息

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.

出版信息

J Virol. 1990 Dec;64(12):5958-65. doi: 10.1128/JVI.64.12.5958-5965.1990.

DOI:10.1128/JVI.64.12.5958-5965.1990
PMID:2173775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248769/
Abstract

We have developed a cell-free system for an avian retrovirus that promotes autointegration, one-long-terminal-repeat (LTR) circle formation, and correct integration into exogenous target DNA. In this system, autointegration and one-LTR circle formation occurred far more frequently than integration into exogenous target DNA. Autointegration had the same characteristics of normal integration into target DNA except in its selection of target. Highly efficient autointegration as well as one-LTR circle formation in vitro suggest that there may be a mechanism to prevent these processes in vivo.

摘要

我们已经开发出一种用于禽逆转录病毒的无细胞系统，该系统可促进自身整合、单长末端重复序列（LTR）环形成以及正确整合到外源靶DNA中。在这个系统中，自身整合和单LTR环形成的发生频率远高于整合到外源靶DNA中。自身整合除了在靶标的选择上与正常整合到靶DNA有所不同外，具有相同的特征。体外高效的自身整合以及单LTR环形成表明，体内可能存在一种阻止这些过程的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/2a3bdbf91a07/jvirol00067-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/bee8421492fd/jvirol00067-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/7e39bf77971a/jvirol00067-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/2e484712a332/jvirol00067-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/ddaca85c7845/jvirol00067-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/2a3bdbf91a07/jvirol00067-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/bee8421492fd/jvirol00067-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/7e39bf77971a/jvirol00067-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/2e484712a332/jvirol00067-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/ddaca85c7845/jvirol00067-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f5/248769/2a3bdbf91a07/jvirol00067-0282-a.jpg

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