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Site-specific nicking at the avian retrovirus LTR circle junction by the viral pp32 DNA endonuclease.

作者信息

Grandgenett D P, Vora A C

出版信息

Nucleic Acids Res. 1985 Sep 11;13(17):6205-21. doi: 10.1093/nar/13.17.6205.

DOI:10.1093/nar/13.17.6205
PMID:2995920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321947/
Abstract

The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases indicates the positions of the two alternative nicked sites. This site-specific nicking was observed when the circle junction LTR DNA was present in supercoiled form, the divalent metal ion was Mg2+ and the molar ratio of protein to DNA was low. The majority of other LTR DNA sites nicked by pp32 in the presence of Mg2+ were adjacent to or within the dinucleotide CA.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/b3c0b8904d9b/nar00311-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/fc2732a07955/nar00311-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/d7d6f89ea7fa/nar00311-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/587e1ee44bb7/nar00311-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/4745dfad8e98/nar00311-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/ea4762fbec1d/nar00311-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/b3c0b8904d9b/nar00311-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/fc2732a07955/nar00311-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/d7d6f89ea7fa/nar00311-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/587e1ee44bb7/nar00311-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/4745dfad8e98/nar00311-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/ea4762fbec1d/nar00311-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e6/321947/b3c0b8904d9b/nar00311-0205-a.jpg

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本文引用的文献

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Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates.由于末端磷酸基团的去除，多脱氧核糖核苷酸在高分辨率聚丙烯酰胺凝胶中的迁移率发生改变。
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Comparison of DNA binding and integration half-site selection by avian myeloblastosis virus integrase.禽成髓细胞瘤病毒整合酶的DNA结合与整合半位点选择的比较
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Avian sarcoma and leukosis virus pol-endonuclease recognition of the tandem long terminal repeat junction: minimum site required for cleavage is also required for viral growth.禽肉瘤和白血病病毒多聚酶-核酸内切酶对串联长末端重复序列连接区的识别：切割所需的最小位点也是病毒生长所必需的。
J Virol. 1987 Jun;61(6):1999-2008. doi: 10.1128/JVI.61.6.1999-2008.1987.
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Characterization of endonuclease activities in Moloney murine leukemia virus and its replication-defective mutants.莫洛尼鼠白血病病毒及其复制缺陷型突变体中的核酸内切酶活性特征
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Nuclease mechanism of the avian retrovirus pp32 endonuclease.禽逆转录病毒pp32核酸内切酶的核酸酶作用机制。
J Virol. 1986 Jun;58(3):970-4. doi: 10.1128/JVI.58.3.970-974.1986.
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J Virol. 1988 Jul;62(7):2358-65. doi: 10.1128/JVI.62.7.2358-2365.1988.
10
Genetic evidence that the avian retrovirus DNA endonuclease domain of pol is necessary for viral integration.有遗传学证据表明，禽逆转录病毒pol基因的DNA核酸内切酶结构域对于病毒整合是必需的。
J Virol. 1988 Jul;62(7):2307-12. doi: 10.1128/JVI.62.7.2307-2312.1988.
Cell. 1984 Mar;36(3):673-9. doi: 10.1016/0092-8674(84)90347-7.
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Nucleic Acids Res. 1984 Mar 26;12(6):2619-28. doi: 10.1093/nar/12.6.2619.
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Avian retrovirus pp32 DNA binding protein. Preferential binding to the promoter region of long terminal repeat DNA.禽逆转录病毒pp32 DNA结合蛋白。与长末端重复序列DNA的启动子区域优先结合。
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6
Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region.糖皮质激素受体与转录区域内及上游位点的MTV DNA发生序列特异性结合。
Cell. 1983 Dec;35(2 Pt 1):381-92. doi: 10.1016/0092-8674(83)90171-x.
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The terminal nucleotides of retrovirus DNA are required for integration but not virus production.逆转录病毒DNA的末端核苷酸是整合所必需的，但不是病毒产生所必需的。
Nature. 1983;306(5939):155-60. doi: 10.1038/306155a0.
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Site-specific DNA condensation and pairing mediated by the int protein of bacteriophage lambda.由噬菌体λ的整合蛋白介导的位点特异性DNA凝聚和配对
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5837-41. doi: 10.1073/pnas.79.19.5837.
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Avian retrovirus pp32 DNA-binding protein. I. Recognition of specific sequences on retrovirus DNA terminal repeats.禽逆转录病毒pp32 DNA结合蛋白。I. 对逆转录病毒DNA末端重复序列上特定序列的识别。
J Virol. 1982 Oct;44(1):330-43. doi: 10.1128/JVI.44.1.330-343.1982.
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Nucleotide sequence of cloned unintegrated avian sarcoma virus DNA: viral DNA contains direct and inverted repeats similar to those in transposable elements.克隆的未整合禽肉瘤病毒DNA的核苷酸序列：病毒DNA含有与转座因子中相似的正向和反向重复序列。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):124-8. doi: 10.1073/pnas.78.1.124.