禽肉瘤病毒感染后鸭胚成纤维细胞胞质及去核细胞中病毒DNA的合成。
Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus.
作者信息
Varmus H E, Guntaka R V, Fan W J, Heasley S, Bishop J M
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3874-8. doi: 10.1073/pnas.71.10.3874.
Abstract
TWO LINES OF EVIDENCE INDICATE THAT SYNTHESIS OF VIRAL DNA OCCURS IN THE CYTOPLASM OF DUCK EMBRYO FIBROBLASTS INFECTED WITH AVIAN SARCOMA VIRUS
(i) viral DNA is detected first in the cytoplasmic fraction of infected cells and subsequently in the nuclear fraction; and (ii) viral DNA is synthesized at a normal rate in cells infected after enucleation with cytochalasin B. The presence of viral DNA in the cytoplasmic fraction is not a consequence of leakage of newly synthesized viral DNA from the nucleus, since it remains in nuclear fractions late after infection when integration of viral DNA into the host genome is inhibited by ethidium bromide.
摘要
有两条证据表明，在感染禽肉瘤病毒的鸭胚成纤维细胞的细胞质中会发生病毒DNA的合成：（i）首先在受感染细胞的细胞质部分检测到病毒DNA，随后在细胞核部分检测到；（ii）在用细胞松弛素B去核后感染的细胞中，病毒DNA以正常速率合成。细胞质部分中病毒DNA的存在并非新合成的病毒DNA从细胞核泄漏的结果，因为在用溴化乙锭抑制病毒DNA整合到宿主基因组后，感染后期病毒DNA仍保留在细胞核部分。
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