克隆的正常3T3细胞、肉瘤病毒转化的3T3细胞及回复突变3T3细胞提取物对DNA合成抑制作用的特异性
Specificity of the inhibition of DNA synthesis by extracts from cloned normal, sarcoma-virus-transformed and revertant 3T3 cells.
作者信息
Ebbesen P, Olsson L
出版信息
Br J Cancer. 1978 Dec;38(6):732-6. doi: 10.1038/bjc.1978.280.
Abstract
Extracts containing tissue-specific DNA-inhibitory activity were prepared from normal FL (Swiss) and BALBc3T3 cells, from these cells transformed with sarcoma virus and from revertants cloned from the transformed cell lines. By testing all extracts on all cell lines we found that (1) production of and susceptibility to the inhibitors were decreased in transformed BALB/c cells (2) specificity varied with expression of the transforming genome, as an extract from a given cell line inhibited the growth of its cell of origin, e.g. revertant, more than normal or transformed cells, and (3) there was also a DNA-synthesis stimulator.
摘要
从正常的FL（瑞士种）和BALBc3T3细胞、用肉瘤病毒转化的这些细胞以及从转化细胞系克隆的回复突变体中制备了含有组织特异性DNA抑制活性的提取物。通过在所有细胞系上测试所有提取物，我们发现：（1）转化的BALB/c细胞中抑制剂的产生和敏感性降低；（2）特异性随转化基因组的表达而变化，例如来自给定细胞系的提取物对其起源细胞（如回复突变体）生长的抑制作用比对正常或转化细胞的抑制作用更强；（3）还存在一种DNA合成刺激剂。
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