微流控 PCR 在即时检测传染病诊断中的进展。

Advances in microfluidic PCR for point-of-care infectious disease diagnostics.

机构信息

Department of Emergency Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

出版信息

Biotechnol Adv. 2011 Nov-Dec;29(6):830-9. doi: 10.1016/j.biotechadv.2011.06.017. Epub 2011 Jun 30.

DOI:10.1016/j.biotechadv.2011.06.017

PMID:21741465

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3186827/

Abstract

Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics.

摘要

现有的或新兴传染病给全球带来的负担，强调了即时检测（POC）诊断的必要性，以增强对疾病的及时识别和干预。基于 PCR 方法的分子方法通过提高检测时间和准确性取得了重大进展，但仍在很大程度上受到集中式实验室资源密集型处理的阻碍，从而排除了其在床边或现场的常规使用。微流控技术已将 PCR 过程小型化到芯片设备上，具有速度快、成本低、便携、高通量和自动化等潜在优势。在这篇综述中，我们概述了微流控 PCR 技术的最新进展，并讨论了将其应用于传染病诊断的实际问题和前景。