Gilligan K, Rajadurai P, Resnick L, Raab-Traub N
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Proc Natl Acad Sci U S A. 1990 Nov;87(22):8790-4. doi: 10.1073/pnas.87.22.8790.
Epstein-Barr virus (EBV) DNA structure and gene expression were analyzed in tissue specimens from oral hairy leukoplakia (HLP), a mucocutaneous lesion that develops in patients infected with human immunodeficiency virus (HIV). The structure of the terminal restriction enzyme fragments of EBV revealed that HLP is a permissive infection without a predominant, detectable population of EBV episomal DNA. In RNA preparations from this uniquely permissive infection, EBV replicative mRNAs could be identified by Northern analysis; however, the virally encoded small nuclear RNAs, the EBERs, were not detected in most HLP RNA preparations. In situ hybridization detected EBER expression in very rare cells. These data indicate that unlike other viral small nuclear RNAs, the EBERs are not expressed during viral replication and must participate in the complex maintenance of latent EBV infection.
在口腔毛状白斑（HLP）的组织标本中分析了爱泼斯坦-巴尔病毒（EBV）的DNA结构和基因表达，HLP是一种在感染人类免疫缺陷病毒（HIV）的患者中出现的黏膜皮肤病变。EBV末端限制性酶切片段的结构显示，HLP是一种允许性感染，没有主要的、可检测到的EBV游离型DNA群体。在这种独特的允许性感染的RNA制剂中，EBV复制性mRNA可通过Northern分析鉴定；然而，在大多数HLP RNA制剂中未检测到病毒编码的小核RNA，即EBERs。原位杂交在非常罕见的细胞中检测到EBER表达。这些数据表明，与其他病毒小核RNA不同，EBERs在病毒复制过程中不表达，且必须参与EBV潜伏感染的复杂维持过程。
