Dambaugh T, Beisel C, Hummel M, King W, Fennewald S, Cheung A, Heller M, Raab-Traub N, Kieff E
Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003. doi: 10.1073/pnas.77.5.2999.
Two of the Sal I fragments and all of the internal BamHI fragments (with the exception of BamHI c, a 0.6 x 10(6) dalton fragment) of Epstein-Barr virus (EBV) DNA have been cloned in pBR322. The termini and other parts of the DNA (including the EcoRI fragment which contains BamHI c) have been cloned as EcoRI fragments in bacteriophage Charon 4A. The cloned DNAs have been used to derive a complete map of the BamHI fragments of EBV DNA and to align the BamHI, EcoRI, HindIII, and SalI cleavage sites in EBV DNA.
爱泼斯坦-巴尔病毒(EBV)DNA的两个Sal I片段以及所有内部BamHI片段(除了BamHI c,一个0.6×10⁶道尔顿的片段)已被克隆到pBR322中。DNA的末端和其他部分(包括含有BamHI c的EcoRI片段)已作为EcoRI片段克隆到噬菌体Charon 4A中。已使用克隆的DNA得出EBV DNA的BamHI片段的完整图谱,并确定EBV DNA中BamHI、EcoRI、HindIII和SalI的切割位点。