Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, Blacksburg, VA 24061-0913, United States.
Vaccine. 2011 Oct 6;29(43):7303-6. doi: 10.1016/j.vaccine.2011.06.097. Epub 2011 Jul 8.
Porcine circovirus type 1 (PCV1), a small DNA virus in pigs, recently gained its notoriety when commercial human rotavirus vaccines were discovered to be contaminated by infectious PCV1. Here we report, for the first time, definitive evidence of productive PCV1 infection in a subclone of human hepatocellular carcinoma cell line (Huh-7, subclone 10-3). Infectious virus was detected in the lysates of infected Huh-7 cells by immunofluorescent assay (IFA) and can be serially passaged in Huh-7-S10-3 cells. The growth kinetic of PCV1 in Huh-7-S10-3 cells was determined in a one-step growth curve using IFA and a quantitative PCR assay. PCV1 achieved a lower infectious titer in Huh-7-S10-3 human cells compared to the titer normally achieved in porcine PK-15 cells from published studies. While the direct relevance to vaccine safety of PCV1 growth in human hepatocellular carcinoma cells is unclear, these data should be considered in further evaluation of vaccines and other products that could contain infectious PCV1.
猪圆环病毒 1 型（PCV1）是一种小型猪 DNA 病毒，最近因其商业用人类轮状病毒疫苗被发现受到传染性 PCV1 污染而声名狼藉。在这里，我们首次报告了在人肝癌细胞系（Huh-7，亚克隆 10-3）的一个亚克隆中发生生产性 PCV1 感染的确凿证据。通过免疫荧光法（IFA）在感染的 Huh-7 细胞的裂解物中检测到了感染性病毒，并可在 Huh-7-S10-3 细胞中连续传代。通过 IFA 和定量 PCR 测定，在一步生长曲线中确定了 PCV1 在 Huh-7-S10-3 细胞中的生长动力学。与已发表的研究中通常在猪 PK-15 细胞中获得的滴度相比，PCV1 在 Huh-7-S10-3 人细胞中的感染滴度较低。虽然 PCV1 在人肝癌细胞中生长与疫苗安全性的直接相关性尚不清楚，但应在进一步评估疫苗和其他可能含有传染性 PCV1 的产品时考虑这些数据。
