Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, 130062, China.
College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
Sci Rep. 2019 Apr 4;9(1):5638. doi: 10.1038/s41598-019-42210-0.
Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy regarding the susceptibility of human cells to PCV2 infection. In this study, human cell lines were infected with PCV2 and blind passaged several times. PCV2 entered and replicated in human cells, and infectious virions were generated, indicating that human cell lines were permissive to PCV2 replication. Furthermore, PCV2 replication in human cell lines was enhanced by D-glucosamine or concanavalin A (ConA). However, the infection efficiency of PCV2 was lower in human cells than in PK-15 cells, suggesting that PCV2 infection was limited in human cells. Our study reveals that human cells are permissive for the productive infection of porcine circovirus type 2 in vitro.
猪圆环病毒 2 型（PCV2）是猪圆环病毒病和猪圆环病毒相关疾病的主要病原体，广泛存在于养猪国家。然而，关于人细胞对 PCV2 感染的易感性存在争议。在本研究中，用人细胞系感染 PCV2 并进行多次盲传。PCV2 进入并在人细胞中复制，并产生感染性病毒粒子，表明人细胞系对 PCV2 复制是允许的。此外，D-葡萄糖胺或刀豆球蛋白 A（ConA）增强了 PCV2 在人细胞系中的复制。然而，PCV2 在人细胞中的感染效率低于 PK-15 细胞，表明 PCV2 感染在人细胞中受到限制。我们的研究表明，人细胞在体外允许猪圆环病毒 2 型的有效感染。
