Soman Sowmya, Rajamanickam Chellam, Rauf Arun A, Indira Madambath
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 695 581, Kerala, India.
Exp Toxicol Pathol. 2013 Jan;65(1-2):91-5. doi: 10.1016/j.etp.2011.06.005. Epub 2011 Jul 13.
Non enzymatic glycosylation (glycation) between reducing sugar and protein results in the formation of advanced glycation end products (AGEs), which is believed to play an important role in diabetes associated cardiovascular complications. Thus agents that inhibit the formation of AGEs are believed to have therapeutic potential against diabetic complications. In the present study we evaluated the antiglycative potential of ethyl acetate fraction of Psidium guajava leaves (PGEt) by administering the extract into streptozotocin induced diabetic rats. Daily administration of the extract for a period of one month significantly decreased the blood glucose, glycated hemoglobin and fructosamine levels in a dose dependent manner. Evaluation of the toxicity markers like SGOT and SGPT revealed the non toxic nature of the extract. Apart from this we evaluated the presence of cardiac isoform of liver alpha 2 macroglobulin, which is a major protein associated with earlier stages of cardiac hypertrophy. SDS-PAGE analysis showed that the level of this protein decreased significantly in extract treated groups compared to diabetic control. These findings support that the administration of PGEt extract may be beneficial for preventing cardiovascular complications associated with diabetes.
还原糖与蛋白质之间的非酶糖基化（糖基化反应）会导致晚期糖基化终末产物（AGEs）的形成，据信其在糖尿病相关心血管并发症中起重要作用。因此，抑制AGEs形成的药物被认为具有治疗糖尿病并发症的潜力。在本研究中，我们通过将番石榴叶乙酸乙酯提取物（PGEt）给予链脲佐菌素诱导的糖尿病大鼠，评估了其抗糖基化潜力。连续一个月每日给予该提取物，能以剂量依赖的方式显著降低血糖、糖化血红蛋白和果糖胺水平。对毒性标志物如谷草转氨酶（SGOT）和谷丙转氨酶（SGPT）的评估显示该提取物无毒性。除此之外，我们还评估了肝脏α2巨球蛋白的心脏同工型的存在情况，它是与心脏肥大早期阶段相关的一种主要蛋白质。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析表明，与糖尿病对照组相比，提取物处理组中这种蛋白质的水平显著降低。这些发现支持给予PGEt提取物可能有利于预防与糖尿病相关的心血管并发症。
