一种灭活的缺失gpX伪狂犬病病毒疫苗的效力
Efficacy of a killed gpX deleted pseudorabies virus vaccine.
作者信息
Cook D R, Hill H T, Kinker D R
机构信息
Veterinary Diagnostic Laboratory, Iowa State University, Ames 50011.
出版信息
Can J Vet Res. 1990 Oct;54(4):438-45.
Ten inactivated vaccines containing one of four adjuvants and varying concentrations of pseudorabies virus (PRV) antigens were compared in order to select a vaccine suitable for commercial production. A genetically engineered strain of PRV lacking the gene coding for glycoprotein X (gpX) was used in these vaccines. Vaccinated pigs were challenged intranasally with virulent PRV to determine the efficacy of vaccines. Vaccination of pigs with one dose of experimental vaccines adjuvanted with 50% Montanide ISA 50 or 20% Syntrogen induced a protective immunity at least equal to that induced by two commercially available killed PRV vaccines also evaluated. An experimental vaccine containing 20% Syntrogen was selected and further evaluated according to United States Department of Agriculture licensing requirements. None of the pigs vaccinated with this vaccine produced gpX antibodies detectable by the HerdChek: Anti-PRV-gpX assay. Therefore, this assay could differentiate PRV vaccine induced antibodies from antibodies induced by natural exposure when used in conjunction with this killed gpX deleted PRV vaccine.
为了选择一种适合商业化生产的疫苗，对十种含有四种佐剂之一和不同浓度伪狂犬病病毒（PRV）抗原的灭活疫苗进行了比较。这些疫苗使用了一种缺乏编码糖蛋白X（gpX）基因的PRV基因工程菌株。给接种疫苗的猪经鼻接种强毒PRV以确定疫苗的效力。用一剂佐以50%Montanide ISA 50或20%Syntrogen的实验疫苗对猪进行接种，诱导出的保护性免疫至少等同于两种也经过评估的市售灭活PRV疫苗所诱导的免疫。选择了一种含有20%Syntrogen的实验疫苗，并根据美国农业部的许可要求进行了进一步评估。用这种疫苗接种的猪均未产生可通过HerdChek：抗PRV - gpX检测法检测到的gpX抗体。因此，当与这种灭活的缺失gpX的PRV疫苗联合使用时，该检测法可以区分PRV疫苗诱导的抗体和自然感染诱导的抗体。
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