de Leeuw P W, van Oirschot J T
Vet Q. 1985 Jul;7(3):191-7. doi: 10.1080/01652176.1985.9693982.
A standardized test was developed to compare the efficacy of Aujeszky's disease virus (ADV) vaccines under laboratory conditions. Per test 3 groups of 6 to 8 sero-negative pigs were used. The first vaccination was done at 10 weeks of age. One group was vaccinated once, another was vaccinated twice and the 3rd served as control. Pigs were challenge exposed to the virulent NIA-3 strain of ADV 12 weeks after the first vaccination. Apart from mortality, average periods of growth arrest, fever and virus shedding after challenge were used as parameters to evaluate vaccine efficacy. Two inactivated and 4 attenuated vaccines were tested. Two attenuated vaccine viruses were excreted after vaccination. Despite maximal standardization, a considerable variation still existed between the experiments in mortality and growth arrest periods of control pigs after challenge. However, the controls were always more severely affected than the vaccinated pigs. All vaccines except one were effective in preventing death after challenge, but none conferred complete protection. Most vaccinated pigs still lost weight, developed fever and shed virus after challenge. Revaccination after 3 or 4 weeks had little effect, particularly with the attenuated vaccines. The results of the present study indicate that 2 of the attenuated vaccines conferred the best protection, 1 attenuated vaccine appeared to be as effective as the 2 inactivated ones, and the 4th attenuated vaccine was least effective.
开发了一种标准化试验，以比较伪狂犬病病毒（ADV）疫苗在实验室条件下的效力。每次试验使用3组6至8头血清阴性猪。首次免疫在10周龄时进行。一组接种一次，另一组接种两次，第三组作为对照。首次接种后12周，猪只接受强毒ADV NIA-3毒株的攻毒。除死亡率外，攻毒后生长停滞、发热和病毒排出的平均持续时间用作评估疫苗效力的参数。测试了2种灭活疫苗和4种弱毒疫苗。接种后排出了2种弱毒疫苗病毒。尽管进行了最大程度的标准化，但攻毒后对照猪的死亡率和生长停滞期在各实验之间仍存在相当大的差异。然而，对照猪总是比接种疫苗的猪受到更严重的影响。除一种疫苗外，所有疫苗在攻毒后均能有效预防死亡，但均未提供完全保护。大多数接种疫苗的猪在攻毒后仍体重减轻、发热并排出病毒。3或4周后再次接种效果甚微，尤其是弱毒疫苗。本研究结果表明，2种弱毒疫苗提供了最佳保护，1种弱毒疫苗似乎与2种灭活疫苗效果相同，第4种弱毒疫苗效果最差。
