College of Animal Sciences, Zhejiang University, Hangzhou, 310029, People's Republic of China.
Mol Biol Rep. 2012 Apr;39(4):3915-23. doi: 10.1007/s11033-011-1170-y. Epub 2011 Jul 10.
Heat shock proteins (HSPs), well-known in respond to various kinds of stress situations, have been widely studied in Drosophila. However, a few reports related to silkworm bombyx mori. Genetic and non-genetic factors affecting on the expression of some HSPs in heat-treated silkworm were studied at the present paper. The mRNA levels of HSPs were quantified by real-time quantitative RT-PCR method and compared with their expression in the proteome profiles. The results showed up-regulation of two small heat shock proteins (sHSPs), HSP19.9 and HSP20.4 and down-regulation of HSP70 in the fat body, testis and ovary of heat exposed larvae. Higher variation of the sHSPs than HSP70 was observed in the different conditions such as heat exposures and genetic backgrounds. Significant difference in the HSP19.9 expression between two breeds was observed which implied the importance of this gene in the genetic differences. There was significant difference between responses of severe and mild heat shocks after 4 h heat recovery. The HSPs expression in male was significantly higher than that in female silkworm larva for all transcript measurements (P < 0.001). Comparison of two methods of quantification showed a fair similarity between HSPs expression in the transcriptome and proteome levels. Nistari breed as a naturally thermo-tolerant breed was expressed lower HSPs than a thermo-sensitive breed.
热休克蛋白（HSPs）在应对各种应激情况方面很有名，在果蝇中已经得到了广泛的研究。然而，关于家蚕的报道却很少。本研究探讨了遗传和非遗传因素对热处理家蚕中一些 HSPs 表达的影响。通过实时定量 RT-PCR 方法定量了 HSPs 的 mRNA 水平，并将其与蛋白质组图谱中的表达进行了比较。结果表明，在受热幼虫的脂肪体、睾丸和卵巢中，两种小热休克蛋白（sHSPs）HSP19.9 和 HSP20.4 的表达上调，HSP70 的表达下调。在不同的条件下，如热暴露和遗传背景下，sHSPs 的变化比 HSP70 更大。在两个品种之间观察到 HSP19.9 表达的显著差异，这表明该基因在家蚕遗传差异中的重要性。在 4 小时热恢复后的严重和轻度热休克反应之间观察到显著差异。对于所有转录测量，雄性家蚕幼虫的 HSPs 表达均显著高于雌性（P<0.001）。两种定量方法的比较表明，转录组和蛋白质组水平的 HSPs 表达具有很好的相似性。Nistari 品种作为一种天然耐热品种，其 HSPs 的表达低于热敏品种。
