Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Inner Mongolia Grassland Station, Hohhot, China.
J Insect Sci. 2019 Nov 1;19(6). doi: 10.1093/jisesa/iez109.
Galeruca daurica (Joannis) has become a new insect pest in the Inner Mongolia grasslands since 2009, and its larvae and eggs have strong cold tolerance. To get a deeper insight into its molecular mechanisms of cold stress responses, we performed de novo transcriptome assembly for G. daurica by RNA-Seq and compared the transcriptomes of its larvae exposed to five different temperature treatments (-10, -5, 0, 5, and 25°C for 1 h and then recovered at 25°C for 1 h), respectively. Compared with the control (25°C), the numbers of differentially expressed genes (DEGs) decreased from 1,821 to 882, with the temperature declining from 5 to -10°C. Moreover, we obtained 323 coregulated DEGs under different low temperatures. Under four low temperatures (-10, -5, 0, and 5°C), a large number of genes were commonly upregulated during recovery from cold stresses, including those related to cuticle protein, followed by cytochrome P450, clock protein, fatty acid synthase, and fatty acyl-CoA reductase; meanwhile, lots of genes encoding cuticle protein, RNA replication protein, RNA-directed DNA polymerase, and glucose dehydrogenase were commonly downregulated. Our findings provide important clues for further investigations of key genes and molecular mechanisms involved in the adaptation of G. daurica to harsh environments.
自 2009 年以来，黄脊竹蝗（Galeruca daurica）（Joannis）已成为内蒙古草原上的一种新的昆虫害虫，其幼虫和卵具有很强的耐寒性。为了更深入地了解其冷应激反应的分子机制，我们通过 RNA-Seq 对黄脊竹蝗进行了从头转录组组装，并比较了暴露于五种不同温度处理（-10、-5、0、5 和 25°C 持续 1 小时，然后在 25°C 恢复 1 小时）的幼虫的转录组。与对照（25°C）相比，随着温度从 5°C 下降到-10°C，差异表达基因（DEGs）的数量从 1821 个减少到 882 个。此外，我们在不同低温下获得了 323 个核心调控的 DEGs。在四个低温（-10、-5、0 和 5°C）下，大量基因在从冷应激中恢复时普遍上调，包括与角质层蛋白相关的基因，其次是细胞色素 P450、时钟蛋白、脂肪酸合酶和脂肪酸酰基辅酶 A 还原酶；同时，大量编码角质层蛋白、RNA 复制蛋白、RNA 指导的 DNA 聚合酶和葡萄糖脱氢酶的基因普遍下调。我们的研究结果为进一步研究黄脊竹蝗适应恶劣环境的关键基因和分子机制提供了重要线索。
