Onuma Kenta, Tanabe Taishi, Sato Hisaaki
Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.
Vet Dermatol. 2012 Feb;23(1):17-22, e5. doi: 10.1111/j.1365-3164.2011.00995.x. Epub 2011 Jul 11.
Staphylococcus pseudintermedius strains were isolated from healthy dogs and dogs with pyoderma in 2000-2002 and 2009. All the isolates from dogs with pyoderma in 1999-2000 and from healthy dogs in 2000-2002 and 2009 were susceptible to cefalexin and/or other cephalosporins and oxacillin. However, 7.1-12.5 and 11.4% of S. pseudintermedius isolates from dogs with pyoderma in 2009 were resistant to cephalosporins and oxacillin, respectively. All S. pseudintermedius isolates from dogs with pyoderma in 1999-2000 and those from healthy dogs in 2000-2002 were susceptible to fluoroquinolones; however, 50% of the S. pseudintermedius strains isolated from dogs with pyoderma in 2009 and 30% of the S. pseudintermedius strains isolated from healthy dogs in 2009 were resistant to fluoroquinolones. Of the 21 oxacillin-resistant S. pseudintermedius (MRSP) isolates, 11 carried SCCmec type V and 10 carried hybrid SCCmec types II-III. Staphylococcus pseudintermedius strains that were resistant to only one of three fluoroquinolones had a mutation in the quinolone resistance determination region of grlA, whereas S. pseudintermedius strains that were resistant to two or more fluoroquinolones had mutations in the quinolone resistance determination regions of both grlA and gyrA.
2000年至2002年以及2009年，从健康犬和患有脓皮病的犬中分离出中间型假葡萄球菌菌株。1999年至2000年患有脓皮病的犬以及2000年至2002年和2009年的健康犬的所有分离株对头孢氨苄和/或其他头孢菌素以及苯唑西林敏感。然而，2009年患有脓皮病的犬的中间型假葡萄球菌分离株分别有7.1%至12.5%和11.4%对头孢菌素和苯唑西林耐药。1999年至2000年患有脓皮病的犬的所有中间型假葡萄球菌分离株以及2000年至2002年健康犬的分离株对氟喹诺酮类药物敏感；然而，2009年患有脓皮病的犬分离出的中间型假葡萄球菌菌株中有50%以及2009年健康犬分离出的中间型假葡萄球菌菌株中有30%对氟喹诺酮类药物耐药。在21株苯唑西林耐药的中间型假葡萄球菌（MRSP）分离株中，11株携带V型葡萄球菌染色体盒式 mec（SCCmec），10株携带II - III型杂交SCCmec。仅对三种氟喹诺酮类药物中的一种耐药的中间型假葡萄球菌菌株在grlA的喹诺酮耐药决定区有突变，而对两种或更多种氟喹诺酮类药物耐药的中间型假葡萄球菌菌株在grlA和gyrA的喹诺酮耐药决定区均有突变。
