Fazakerley Jennifer, Williams Nicola, Carter Stuart, McEwan Neil, Nuttall Tim
The University of Liverpool School of Veterinary Science, Small Animal Teaching Hospital, Leahurst Campus, Neston, Cheshire CH64 7TE, UK.
Vet Dermatol. 2010 Dec;21(6):578-85. doi: 10.1111/j.1365-3164.2010.00894.x.
Staphylococcus pseudintermedius is part of the normal canine flora but frequently causes pyoderma in canine atopic dermatitis (AD). This study aimed to determine whether particular S. pseudintermedius strains were associated with AD and/or pyoderma. Ninety-six S. pseudintermedius isolates from the ear, nares, perineum and lesions of 21 atopic and 16 healthy dogs were lysed with proteinase K and digested with 40 U SmaI. Restriction products were separated using pulsed-field gel electrophoresis (PFGE) with an Oxford S. aureus control and lambda-ladder DNA concatomer markers. A dendrogram was constructed by the unweighted pair group method. All isolates showed a ≥ 56% similarity coefficient. Nine distinct PFGE clusters were identified, as follows: five from both atopic and healthy dogs; three from atopic dogs only; and one from healthy dogs only. Nine clusters were isolated from the nares, eight from the perineum, five from the ears and six from pyoderma lesions. There were no significant differences in the frequency of isolation from atopic or healthy skin, body sites or infected lesions for any of the clusters. Two of six healthy dogs and 18 of 20 atopic dogs with multiple isolates had closely related isolates (less than three band differences) at more than one sampling site. Isolates from pyoderma lesions were closely related to at least one mucosal isolate in 11 of 16 dogs. Staphylococcus pseudintermedius isolates appear to be heterogeneous, and colonization or infection of atopic skin was not associated with any particular strain or cluster of strains.
中间型假丝酵母葡萄球菌是犬正常菌群的一部分，但在犬特应性皮炎（AD）中常引起脓皮病。本研究旨在确定特定的中间型假丝酵母葡萄球菌菌株是否与AD和/或脓皮病相关。从21只特应性犬和16只健康犬的耳朵、鼻孔、会阴和病变部位分离出96株中间型假丝酵母葡萄球菌，用蛋白酶K裂解，并用40 U SmaI消化。使用脉冲场凝胶电泳（PFGE），以牛津金黄色葡萄球菌为对照和λ-阶梯DNA串联体标记物，分离限制性产物。采用非加权配对组法构建树状图。所有分离株显示相似系数≥56%。鉴定出9个不同的PFGE簇，如下：5个来自特应性犬和健康犬；3个仅来自特应性犬；1个仅来自健康犬。从鼻孔分离出9个簇，从会阴分离出8个，从耳朵分离出5个，从脓皮病病变分离出6个。任何簇在特应性或健康皮肤、身体部位或感染病变中的分离频率均无显著差异。在有多个分离株的6只健康犬中有2只，20只特应性犬中有18只在多个采样部位有密切相关的分离株（条带差异少于3条）。在16只犬中的11只中，脓皮病病变分离株与至少一种黏膜分离株密切相关。中间型假丝酵母葡萄球菌分离株似乎具有异质性，特应性皮肤的定植或感染与任何特定菌株或菌株簇无关。
