Beck Karri M, Waisglass Stephen E, Dick Hani L N, Weese J Scott
Veterinary Emergency Clinic and Referral Centre, 920 Yonge Street Suite 117, Toronto, Ontario M4W 3C7, Canada.
Vet Dermatol. 2012 Aug;23(4):369-75, e66-7. doi: 10.1111/j.1365-3164.2012.01035.x. Epub 2012 Feb 24.
Meticillin-resistant staphylococci are significant pathogens in veterinary dermatology, yet longitudinal studies of the impact of routine antimicrobial therapy on emergence or resolution of resistance are lacking.
To determine the prevalence of meticillin-resistant staphylococci on skin and carriage sites in dogs with bacterial pyoderma and evaluate the prevalence of meticillin-resistant Staphylococcus pseudintermedius (MRSP) colonization after successful treatment of pyoderma.
One hundred and seventy-three dogs that presented to a dermatology referral service with pyoderma and 41 healthy control dogs.
Skin, nasal and rectal swabs for bacterial culture were collected at the time of referral and after clinical resolution of the pyoderma. Meticillin resistance was confirmed by demonstration of penicillin binding protein 2a antigen.
Initially, skin cultures yielded MRSP in 70 (40.5%) dogs, meticillin-resistant Staphylococcus aureus (MRSA) in three (1.7%) and meticillin-resistant Staphylococcus schleiferi ssp. coagulans (MRSScoag) in five (2.9%). Samples collected from the nose and rectum (carriage sites) yielded MRSP in 59 (34.1%) dogs, MRSA in 11 (6.4%) and MRSScoag in seven (4.0%). One hundred and two dogs were available for follow-up cultures after clinical cure. Of 42 dogs initially diagnosed with MRSP pyoderma, MRSP was isolated at follow-up from skin in 19 (45.2%) and carriage sites in 20 (47.6%). Of 60 dogs that did not have MRSP pyoderma initially, MRSP was isolated post-treatment from the skin in 17 (28.3%), and MRSP from carriage sites increased from 7.8% (initially) to 26.7% (P = 0.0022).
Colonization by MRSP often persists after resolution of MRSP pyoderma. Acquisition of MRSP during treatment appears to be common.
耐甲氧西林葡萄球菌是兽医皮肤病学中的重要病原体，但缺乏关于常规抗菌治疗对耐药性出现或消退影响的纵向研究。
确定患有细菌性脓皮病的犬皮肤和携带部位耐甲氧西林葡萄球菌的患病率，并评估脓皮病成功治疗后耐甲氧西林中间型葡萄球菌（MRSP）定植的患病率。
173只因脓皮病就诊于皮肤科转诊服务机构的犬和41只健康对照犬。
在转诊时和脓皮病临床消退后采集皮肤、鼻腔和直肠拭子进行细菌培养。通过检测青霉素结合蛋白2a抗原确认甲氧西林耐药性。
最初，皮肤培养在70只（40.5%）犬中检出MRSP，3只（1.7%）检出耐甲氧西林金黄色葡萄球菌（MRSA），5只（2.9%）检出耐甲氧西林施氏葡萄球菌凝固酶亚种（MRSScoag）。从鼻子和直肠（携带部位）采集的样本在59只（34.1%）犬中检出MRSP，11只（6.4%）检出MRSA，7只（4.0%）检出MRSScoag。102只犬在临床治愈后可进行随访培养。最初诊断为MRSP脓皮病的42只犬中，随访时19只（45.2%）皮肤分离出MRSP，20只（47.6%）携带部位分离出MRSP。最初没有MRSP脓皮病的60只犬中，治疗后17只（28.3%）皮肤分离出MRSP，携带部位的MRSP从7.8%（最初）增加到26.7%（P = 0.0022）。
MRSP脓皮病消退后，MRSP定植通常持续存在。治疗期间获得MRSP似乎很常见。
