Han Xue, Wu Xia, Liu Hongchen, Wang Dongsheng, E Lingling, Zhou Wei
Department of Stomatology, 309th Hospital of Chinese People's Liberation Army, Beijing, 100091, China.
J Mater Sci Mater Med. 2015 Jan;26(1):5338. doi: 10.1007/s10856-014-5338-6. Epub 2015 Jan 11.
An injectable bone cement, nHAC/CSH, which consists of nano-hydroxyapatite/collagen (nHAC) and calcium sulphate hemihydrate (CaSO4.½H2O; CSH) was investigated as a tissue-engineered scaffold material with blood-acquired mesenchymal progenitor cells (BMPCs) as seeding cells. An in vitro study on the cytocompatability of nHAC/CSH and an in vivo study on the ectopic bone formation of nHAC/CSH loaded with dBMPCs were both conducted. The dBMPCs morphology, proliferation, differentiation and apoptosis assays were conducted using the direct contact and extract method. The cells tests exhibited normal growth and bioactive function in vitro. Studies in vivo showed that this injectable tissue engineered bone (ITB) formed bone structure in the heterotopic site of nude mice. These findings indicate that the ITB composed of nHAC/CSH and dBMPCs may represent a useful strategy for clinical reconstruction of irregular bone defects.
一种可注射骨水泥，即纳米羟基磷灰石/半水硫酸钙（nHAC/CSH），它由纳米羟基磷灰石/胶原蛋白（nHAC）和半水硫酸钙（CaSO4·½H2O；CSH）组成，被作为一种组织工程支架材料进行研究，以血液来源的间充质祖细胞（BMPCs）作为种子细胞。对nHAC/CSH的细胞相容性进行了体外研究，并对负载有dBMPCs的nHAC/CSH的异位骨形成进行了体内研究。使用直接接触和提取物方法进行了dBMPCs的形态、增殖、分化和凋亡检测。细胞测试表明其在体外生长正常且具有生物活性功能。体内研究表明，这种可注射组织工程骨（ITB）在裸鼠的异位部位形成了骨结构。这些发现表明，由nHAC/CSH和dBMPCs组成的ITB可能是临床重建不规则骨缺损的一种有用策略。
