邻苯二甲酸二（2-乙基己基）酯对大鼠不同组织中脂肪酸氧化及肉碱棕榈酰转移酶的影响

The effect of di(ethylhexyl)phthalate on fatty acid oxidation and carnitine palmitoyltransferase in various rat tissues.

作者信息

Reubsaet F A, Veerkamp J H, Dirven H A, Brückwilder M L, Hashimoto T, Trijbels J M, Monnens L A

机构信息

Department of Pediatrics, University of Nijmegen, The Netherlands.

出版信息

Biochim Biophys Acta. 1990 Dec 4;1047(3):264-70. doi: 10.1016/0005-2760(90)90525-3.

DOI:10.1016/0005-2760(90)90525-3

PMID:2174704

Abstract

Male rats were fed a diet with or without 2% di(2-ethylhexyl)phthalate (DEHP) for 12 days. Total and peroxisomal oxidation rates of palmitic and arachidonic acid were increased in homogenates of liver and kidney after DEHP administration. The relative peroxisomal contribution to the total oxidation was only higher in liver. The activities of acyl-CoA oxidase and carnitine palmitoyltransferase were also higher in both tissues. Immunoblots showed that the increase of fatty acid oxidation was associated with a higher concentration of enzymes of peroxisomal and mitochondrial beta-oxidation. DEHP did not change total and peroxisomal fatty acid oxidation and activity of carnitine palmitoyltransferase of homogenates of heart and skeletal muscle. The cause for the tissue-specific response is discussed.

摘要

将雄性大鼠分为两组，一组喂食含2%邻苯二甲酸二（2-乙基己基）酯（DEHP）的饲料，另一组喂食不含DEHP的饲料，持续12天。给予DEHP后，肝脏和肾脏匀浆中棕榈酸和花生四烯酸的总氧化率和过氧化物酶体氧化率均升高。过氧化物酶体对总氧化的相对贡献仅在肝脏中较高。两种组织中酰基辅酶A氧化酶和肉碱棕榈酰转移酶的活性也较高。免疫印迹显示，脂肪酸氧化的增加与过氧化物酶体和线粒体β-氧化酶的浓度升高有关。DEHP对心脏和骨骼肌匀浆的总脂肪酸氧化、过氧化物酶体脂肪酸氧化以及肉碱棕榈酰转移酶的活性没有影响。文中讨论了组织特异性反应的原因。