Wong Ming-Ching, Martynovsky Maria, Schwarzbauer Jean E
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Methods Mol Biol. 2011;769:233-47. doi: 10.1007/978-1-61779-207-6_16.
The nematode Caenorhabditis elegans is an excellent model system in which to study long-distance cell migration in vivo. This chapter describes methods used to study a subset of migratory cells in the hermaphrodite nematode, the distal tip cells. These methods take advantage of the organism's transparent body and the expression of green fluorescent protein to observe cell migration and behavior. Additionally, the availability of nematode mutants and gene knockdown techniques that affect cell migration allow the analysis and comparison of wild-type and aberrant migratory paths. Methods for nematode growth and maintenance, strain acquisition, observation and live imaging, gene knockdown, and analysis of cell migration defects are covered.
线虫秀丽隐杆线虫是研究体内远距离细胞迁移的优秀模型系统。本章描述了用于研究雌雄同体线虫中一部分迁移细胞——远端末梢细胞的方法。这些方法利用了该生物体透明的身体以及绿色荧光蛋白的表达来观察细胞迁移和行为。此外，线虫突变体和影响细胞迁移的基因敲低技术的可用性使得对野生型和异常迁移路径进行分析和比较成为可能。文中涵盖了线虫生长与维持、菌株获取、观察与实时成像、基因敲低以及细胞迁移缺陷分析等方法。
