Meighan Christopher M, Schwarzbauer Jean E
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Genes Dev. 2007 Jul 1;21(13):1615-20. doi: 10.1101/gad.1534807.
Integrin receptors for extracellular matrix are critical for cell motility, but the signals that determine when to stop are not known. Analysis of distal tip cell (DTC) migration during gonadogenesis in Caenorhabditis elegans has revealed the importance of transcription factor vab-3/Pax6 in regulating the alpha integrin genes, ina-1 and pat-2. Utilizing vab-3 mutants, we show that the down-regulation of ina-1 is necessary for DTC migration cessation and the up-regulation of pat-2 is required for directionality. These results demonstrate concomitant, but distinct roles in migration for each integrin. Notably, transcriptional control of migration termination provides a new mechanism for regulation of morphogenesis and organ size.
细胞外基质的整合素受体对细胞运动至关重要,但决定何时停止运动的信号尚不清楚。对线虫性腺发育过程中远端末梢细胞(DTC)迁移的分析揭示了转录因子vab-3/Pax6在调节α整合素基因ina-1和pat-2中的重要性。利用vab-3突变体,我们发现ina-1的下调是DTC迁移停止所必需的,而pat-2的上调是定向迁移所必需的。这些结果表明,每种整合素在迁移中具有伴随但不同的作用。值得注意的是,迁移终止的转录控制为形态发生和器官大小的调节提供了一种新机制。