Cram Erin J, Shang Hongyu, Schwarzbauer Jean E
Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA.
J Cell Sci. 2006 Dec 1;119(Pt 23):4811-8. doi: 10.1242/jcs.03274. Epub 2006 Nov 7.
Cell migration is essential during embryonic development and tissue morphogenesis. During gonadogenesis in the nematode Caenorhabditis elegans, migration of the distal tip cells forms two U-shaped gonad arms. Malformation results if the distal tip cells stop prematurely or follow an aberrant path, and abnormalities are easily visualized in living nematodes. Here we describe the first comprehensive in vivo RNA interference screen for genes required for cell migration. In this non-biased screen, we systematically analyzed 16,758 RNA-interference depletion experiments by light microscopy and identified 99 genes required for distal tip cell migration. Genetic and physical interaction data connect 59 of these genes to form a cell migration gene network that defines distal tip cell migration in vivo.
细胞迁移在胚胎发育和组织形态发生过程中至关重要。在线虫秀丽隐杆线虫的性腺发生过程中，远端顶端细胞的迁移形成了两个U形性腺臂。如果远端顶端细胞过早停止迁移或沿着异常路径迁移，就会导致畸形，并且在活体线虫中很容易观察到异常情况。在这里，我们描述了第一个针对细胞迁移所需基因的全面体内RNA干扰筛选。在这个无偏向性的筛选中，我们通过光学显微镜系统地分析了16758个RNA干扰缺失实验，并鉴定出99个远端顶端细胞迁移所需的基因。遗传和物理相互作用数据将其中59个基因连接起来，形成了一个在体内定义远端顶端细胞迁移的细胞迁移基因网络。
