Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Am J Reprod Immunol. 2013 Feb;69(2):134-41. doi: 10.1111/aji.12030. Epub 2012 Oct 11.
Local adaptive cervical regulatory T cells (Tregs) are the most likely direct suppressors of the immune eradication of cervical intraepithelial lesion (CIN). PD-1 expression on T cells induces Tregs. No studies have quantitatively analyzed the Tregs and PD-1+ cells residing in CIN lesions.
Cervical lymphocytes were collected using cytobrushes from CIN patients and analyzed by FACS analysis. Comparisons were made between populations of cervical Tregs and PD-1+ CD4+ T cells in CIN regressors and non-regressors.
A median of 11% of cervical CD4+ T cells were Tregs, while a median of 30% were PD-1+ cells. The proportions of cervical CD4+ T cells that were Tregs and/or PD-1+ cells were significantly lower in CIN regressors when compared with non-regressors.
The prevalence of cervical tolerogenic T cells correlates inversely with spontaneous regression of CIN. Cervical Tregs may play an important role in HPV-related neoplastic immunoevasion.
局部适应性宫颈调节性 T 细胞（Tregs）最有可能直接抑制宫颈上皮内瘤变（CIN）的免疫清除。T 细胞上的 PD-1 表达诱导 Tregs。目前尚无研究定量分析 CIN 病变中存在的 Tregs 和 PD-1+细胞。
使用 cytobrushes 从 CIN 患者中收集宫颈淋巴细胞，并通过 FACS 分析进行分析。比较 CIN 消退者和非消退者中宫颈 Tregs 和 PD-1+CD4+T 细胞的群体。
中位数为 11%的宫颈 CD4+T 细胞为 Tregs，而中位数为 30%的为 PD-1+细胞。与非消退者相比，CIN 消退者宫颈 CD4+T 细胞中 Tregs 和/或 PD-1+细胞的比例显著降低。
宫颈耐受 T 细胞的流行与 CIN 的自发消退呈负相关。宫颈 Tregs 可能在 HPV 相关肿瘤免疫逃逸中发挥重要作用。
