Costanzo G, Di Mauro E, Salina G, Negri R
Centro Acidi Nucleici (CNR), Rome, Italy.
J Mol Biol. 1990 Nov 20;216(2):363-74. doi: 10.1016/S0022-2836(05)80327-4.
Nucleosome core particles were reconstituted on various DNA fragments containing a Crithidia fasciculata kinetoplast curved tract. The results show that, on curved DNA, nucleosome core particles form six- to sevenfold preferentially, relative to bulk sequences. The preferential deposition occurs at multiple periodic positions, whose distribution reveals a unique rotational setting of DNA with respect to the histone octamer surface and whose average periodicity is 10.26 +/- 0.04. Evidence is provided for a context effect in histone octamer deposition: octamers bound to a segment of curved DNA influence the positions of neighbour octamers. Taken together, the preferential formation of nucleosome core particles and the influence on the localization of neighbouring particles suggest for intrinsically bent sequences the biologically relevant role of organizers of nucleosomal arrays.
核小体核心颗粒在含有克氏锥虫动质体弯曲序列的各种DNA片段上进行了重构。结果表明，相对于整体序列，在弯曲DNA上，核小体核心颗粒优先以六到七倍的比例形成。优先沉积发生在多个周期性位置，其分布揭示了DNA相对于组蛋白八聚体表面的独特旋转取向，其平均周期为10.26±0.04。有证据表明组蛋白八聚体沉积存在背景效应：与一段弯曲DNA结合的八聚体会影响相邻八聚体的位置。综合来看，核小体核心颗粒的优先形成以及对相邻颗粒定位的影响表明，对于内在弯曲序列，核小体阵列组织者具有生物学相关作用。
