Buttinelli M, Negri R, Di Marcotullio L, Di Mauro E
Centro Acidi Nucleici, Consiglio Nazionale delle Ricerche, Università di Roma La Sapienza, Italy.
Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10747-51. doi: 10.1073/pnas.92.23.10747.
The effects of the rotational information of DNA in determining the in vitro localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5S rRNA repeat gene. We have altered the distribution of the phased series of flexibility signals present on this DNA by inserting a 25-bp tract, and we have analyzed the effects of this mutation on the distribution and on the frequencies of ncps, as compared with the wild type and a reference 21-bp insertion mutant. The variation of the standard free energy of nucleosome reconstitution was determined. The results show that the DNA rotational information is a major determinant of ncps positioning, define how many rotationally phased signals are required for the formation of a stable particle, and teach how to modify their distribution through the alteration of the rotational signals.
在酿酒酵母5S rRNA重复基因中，研究了DNA的旋转信息在确定核小体核心颗粒（ncps）体外定位方面的作用。我们通过插入一段25个碱基对的序列，改变了该DNA上存在的相位系列柔性信号的分布，并与野生型和一个参考21个碱基对插入突变体相比，分析了这种突变对ncps分布和频率的影响。测定了核小体重构标准自由能的变化。结果表明，DNA旋转信息是ncps定位的主要决定因素，确定了形成稳定颗粒所需的旋转相位信号数量，并表明如何通过改变旋转信号来改变其分布。
